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MB Sample ID: SA308721
Local Sample ID: | N_EF-B-3 |
Subject ID: | SU002966 |
Subject Type: | Bacteria |
Subject Species: | Staphylococcus aureus/ Acinetobacter baumannii/ Enterococcus faecium/ Pseudomonas aeruginosa |
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Combined analysis:
Analysis ID | AN004675 | AN004676 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Waters Acquity | Waters Acquity |
Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Waters Synapt G2 XS QTOF | Waters Synapt G2 XS QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | internsity | intensity |
MS:
MS ID: | MS004422 |
Analysis ID: | AN004675 |
Instrument Name: | Waters Synapt G2 XS QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | The UPLC was connected to the electrospray ionization source of the traveling wave ion mobility-mass spectrometer (Waters Synapt XS) and samples were injected at 5 uL. Prior to acquisition of sample data, data was acquired for a mixture of CCS calibrants using direct infusion. Randomized sample queues were analyzed in both positive and negative ionization modes. A pooled mixture of all samples was used as a quality control (QC). Data was collected across the entire 17 min chromatographic method using data-independent MS/MS acquisition. Leucine enkephalin was monitored for post-acquisition lockmass correction. Capillary +3 kV; Sampling Cone 30 V; Sampling Cone 25 V; Source Offset 40 V; Source Temp 150 ºC; Desolvation Temp 400 ºC; Cone gas flow 50 L/h; Desolvation gas flow 650 L/h; Nebulizer gas flow 7 Bar. Mass Range 50-1200 m/z. |
Ion Mode: | POSITIVE |
MS ID: | MS004423 |
Analysis ID: | AN004676 |
Instrument Name: | Waters Synapt G2 XS QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | The UPLC was connected to the electrospray ionization source of the traveling wave ion mobility-mass spectrometer (Waters Synapt XS) and samples were injected at 5 uL. Prior to acquisition of sample data, data was acquired for a mixture of CCS calibrants using direct infusion. Randomized sample queues were analyzed in both positive and negative ionization modes. A pooled mixture of all samples was used as a quality control (QC). Data was collected across the entire 17 min chromatographic method using data-independent MS/MS acquisition. Leucine enkephalin was monitored for post-acquisition lockmass correction. Capillary -2 kV; Sampling Cone 30 V; Sampling Cone 25 V; Source Offset 40 V; Source Temp 150 ºC; Desolvation Temp 400 ºC; Cone gas flow 50 L/h; Desolvation gas flow 650 L/h; Nebulizer gas flow 7 Bar. Mass Range 50-1200 m/z. |
Ion Mode: | NEGATIVE |