Return to study ST002910 main page
MB Sample ID: SA316293
| Local Sample ID: | MDA03-01-4742 |
| Subject ID: | SU003023 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004779 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Bruker Elute |
| Column | Hamilton Intensity Solo 2 C18 (100 mm x 2.1 mm, 1.8um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Bruker timsTOF |
| Ion Mode | POSITIVE |
| Units | AU |
MS:
| MS ID: | MS004525 |
| Analysis ID: | AN004779 |
| Instrument Name: | Bruker timsTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Using an Apollo II electrospray ionization (ESI) source or an CaptiveSpray ion source, and a timsTOF (Bruker, Bremen, Germany), the MS analysis was carried out. For metabolomics, the nebulizer pressure was adjusted to 2.2 bar, the drying temperature to 220°C, and the gas flow rate to 10 L/min. 4500 V was the capillary voltage, while 500 V was the end plate offset and the scan range for metabolomics was 20-1300 m/z. The instrument was operated in auto-MS/MS mode and the acquisition was performed using the positive mode at 12 Hz. The width of the precursor ion was ±0.5, the cycle time was 0.5 sec., and the threshold was 400 counts per thousand. Active exclusion was excluded after 3 spectra and released after 0.2 min. For MS2 acquisition the data dependent acquisition (DDA) was used, and the collision energy stepping fluctuated between 100 and 250% set at 20 eV. In the first 0.3 minutes of each LC-MS/MS run, sodium formate was injected as an external calibrant during data processing |
| Ion Mode: | POSITIVE |
