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MB Sample ID: SA316707
| Local Sample ID: | 20120522-zhxl-A4-L-1 |
| Subject ID: | SU003031 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004788 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Thermo Scientific Trace GC Ultra with DSQ II GC/MS |
| Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Scientific Trace GC Ultra with DSQ II GC/MS |
| Ion Mode | POSITIVE |
| Units | Peak area |
MS:
| MS ID: | MS004534 |
| Analysis ID: | AN004788 |
| Instrument Name: | Thermo Scientific Trace GC Ultra with DSQ II GC/MS |
| Instrument Type: | Triple quadrupole |
| MS Type: | EI |
| MS Comments: | samples was derivatized and then used to firstly protect carbonyl moieties through methoximation, through a 90 min 37 ℃ reaction with 40 μL of 20 mg/mL methoxyamine hydrochloride (Sigma-Aldrich) in pyridine, followed by derivatization of acidic protons through a 30 min 37 0C reaction with the addition of 80 μL N-methyl-N-trimethylsilyltrifluoroace-tamide (MSTFA, Sigma-Aldrich). The derivatized sample of 1 μL was injected into a 30m × 250 μm i.d. × 0.25 μm DBS-MS column using splitless injection and analysis was carried out by Trace DSQ II (Thermo Scientific). The initial temperature of the GC oven was held at 85 0C for 5 min followed by an increase to 330 0C at a rate of 15 0C min-1 then held for 5 min. Helium was used as carrier gas and flow was kept constant at 1 mL min-1. The MS was operated in a range of 50-600 m/z. |
| Ion Mode: | POSITIVE |
