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MB Sample ID: SA317494

Local Sample ID:AD_FA_83
Subject ID:SU003036
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:infant
Gender:Not applicable
Human Race:Pacific asian

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Combined analysis:

Analysis ID AN004793
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000
Column Phenomenex Jupiter C4 (50 × 1.0 mm, 5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo LTQ XL
Ion Mode NEGATIVE
Units pmol/mg

MS:

MS ID:MS004539
Analysis ID:AN004793
Instrument Name:Thermo LTQ XL
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Selected ion monitoring (SRM) was uesd in the negative ion mode and the extracted ion chromatogram corresponding to the specific transition (phosphate ion, PO3-, m/z 78.9585) for S1P was used for quantification. Calibration range was 0.01 – 10 μM with R2 > 0.99. Data analysis was performed by using Xcalibur 2.2 software. Ultimate3000
Ion Mode:NEGATIVE
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