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MB Sample ID: SA323713

Local Sample ID:HSCb_14
Subject ID:SU003096
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Combined analysis:

Analysis ID AN004902
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system none
Column none
MS Type MALDI
MS instrument type MALDI-TOF-MS
MS instrument name Bruker Autoflex MALDI-TOF (/TOF)-MS
Ion Mode POSITIVE
Units intensity

MS:

MS ID:MS004645
Analysis ID:AN004902
Instrument Name:Bruker Autoflex MALDI-TOF (/TOF)-MS
Instrument Type:MALDI-TOF-MS
MS Type:MALDI
MS Comments:MS acquisition Comments: MS experiments were conducted on the Autoflex MALDI-TOF (/TOF)-MS (Bruker Autoflex Speed) with Nd:YAG lasers (355 nm) and smart beam system. All MS experiments were performed in the reflector positive ion mode, with 2000 laser shots per analysis and 5 independent experiments per sample. Data processing Comments:MS data preprocessing was carried out on python version 3.8, including peak detection, peak filtration, standardization, and batch effect removal by ComBat algorithm. The heatmap, hierarchical clustering analysis, Pearson correlation analysis and PCA analysis were performed on MetaboAnalyst 5.0 (https://www.metaboanalyst.ca/). Machine learning and t-SNE clustering were conducted on Orange (version 3.25.0, the Bioinformatics Lab at University of Ljubljana, Slovenia). Software/procedures used for feature assignments: Python
Ion Mode:POSITIVE
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