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MB Sample ID: SA326888

Local Sample ID:EV_24h_C_neg_1
Subject ID:SU003121
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090

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Combined analysis:

Analysis ID AN004938
Analysis type MS
Chromatography type HILIC
Chromatography system Vanquish Horizon
Column Waters ACQUITY UPLC BEH Amide (100 × 2.1mm, 1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo orbitrap exploris 240
Ion Mode NEGATIVE
Units Peak area

MS:

MS ID:MS004681
Analysis ID:AN004938
Instrument Name:Thermo orbitrap exploris 240
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:ESI source parameters were set as follows: spray voltage, 3500 V or −2800 V, in positive or negative modes, respectively; vaporizer temperature, 350 °C; sheath gas, 50 arb; aux gas, 10 arb; ion transfer tube temperature, 325 °C. The full scan was set as: orbitrap resolution, 60,000; maximum injection time, 100 ms; scan range, 70–1050 Da. The ddMS2 scan was set as: orbitrap resolution, 30,000; maximum injection time, 60 ms; top N setting, 6; isolation width, 1.0 m/z; HCD collision energy (%), 30; Dynamic exclusion mode was set as auto. The metabolites was quantified by Compound Discoverer 3.3.
Ion Mode:NEGATIVE
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