Return to study ST003017 main page
MB Sample ID: SA327634
| Local Sample ID: | Sp03 |
| Subject ID: | SU003131 |
| Subject Type: | Mammal |
| Subject Species: | Florida manatee (Trichechus manatus latirostris) |
| Age Or Age Range: | Adult |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004950 | AN004951 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC BEH C18(100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18(100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
MS:
| MS ID: | MS004690 |
| Analysis ID: | AN004950 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All samples were analyzed in full scan positive and negative ion modes with spray voltages +3.0kV and -3.0kV, respectively, at 70,000 resolution at m/z 200, the scan range was m/z 100-1500 and source temperature at 300 °C. Tandem mass spectra were collected via criterion based on data-dependent acquisition for the top-10 ions with stepped normalized collision energy 20, 25, and 30, an isolation window of 1Da, dynamic exclusion of 6 s, and using IE-Omics for the generation of exclusion lists in iterative-exclusion experiments (Koelmel, Kroeger, Gill, et al., 2017). Extracted Lipid Data Analysis LipidMatch software was implemented to identify and integrate the detected lipids (Koelmel, Kroeger, Ulmer, et al., 2017). The peak areas were normalized relative to the internal standards of the same subclass as the lipid of interest. For lipids that did not have an internal standard of the same subclass, the internal standard with the closest chemical structure was used (i.e., internal standard with the same head group). Otherwise, the internal standard with the closest chromatographic retention time (same ionization polarity) was used for analytes without a corresponding internal standard of the same lipid subclass. The plasma lipid concentrations were reported as µg/mL. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | MS Metadata.txt |
| MS ID: | MS004691 |
| Analysis ID: | AN004951 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All samples were analyzed in full scan positive and negative ion modes with spray voltages +3.0kV and -3.0kV, respectively, at 70,000 resolution at m/z 200, the scan range was m/z 100-1500 and source temperature at 300 °C. Tandem mass spectra were collected via criterion based on data-dependent acquisition for the top-10 ions with stepped normalized collision energy 20, 25, and 30, an isolation window of 1Da, dynamic exclusion of 6 s, and using IE-Omics for the generation of exclusion lists in iterative-exclusion experiments (Koelmel, Kroeger, Gill, et al., 2017). Extracted Lipid Data Analysis LipidMatch software was implemented to identify and integrate the detected lipids (Koelmel, Kroeger, Ulmer, et al., 2017). The peak areas were normalized relative to the internal standards of the same subclass as the lipid of interest. For lipids that did not have an internal standard of the same subclass, the internal standard with the closest chemical structure was used (i.e., internal standard with the same head group). Otherwise, the internal standard with the closest chromatographic retention time (same ionization polarity) was used for analytes without a corresponding internal standard of the same lipid subclass. The plasma lipid concentrations were reported as µg/mL. |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | MS Metadata.txt |
