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MB Sample ID: SA330839
| Local Sample ID: | WT_C |
| Subject ID: | SU003162 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004998 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 8890 |
| Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | GC-Q-MS |
| MS instrument name | Agilent 5977B |
| Ion Mode | POSITIVE |
| Units | relative abundance |
MS:
| MS ID: | MS004738 |
| Analysis ID: | AN004998 |
| Instrument Name: | Agilent 5977B |
| Instrument Type: | GC-Q-MS |
| MS Type: | EI |
| MS Comments: | Cell homogenates were prepared by adding 1:1 v/v water: methanol and sonicated with a dr.hielscher UP200S (dr.hielscher, Berlin, Germany), set up to 80% intensity, 0.5 s/pulse, 16 pulses. For GC-MS, 100 μL of each homogenate was mixed with 300 μL of cold methanol containing 25 ppm D-palmitic acid (internal standard), vortex mixed for 60 min and centrifuged at 4000 g for 20 min. 250 μL of the supernatant were transferred to a vial with a glass insert and evaporated to dryness in a Gyrozen HyperVac VC2124 (Gimpo, Korea) coupled to a LVS 110Z (Gardner Denver Thomas GmbH Welch Vacuum, Fürstenfeldbruck, Germany). The samples were then submitted to methoximation (with O-methoxyamine) for 16 h and silylation for 1 h at 70ºC with N,O-Bis(trime5j,thylsilyl)trifluoroacetamide (BSTFA) and trimethylchlorosilane (TCMS), and finally resuspended in 100 μL heptane. The samples were injected onto a DB5-MS column (30 m x 0.250 mm, 0.25 μm) with a 10 m pre-column (Agilent Technologies, Santa Clara CA, USA). Helium was used as mobile phase at constant flow rate (1 mL/min) in a GC-Q-MS 5975 system (Agilent Technologies) with an Electron Ionization (EI) source at 70 eV. |
| Ion Mode: | POSITIVE |
