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MB Sample ID: SA341049
| Local Sample ID: | STD8_a |
| Subject ID: | SU003271 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005174 | AN005175 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | GC |
| Chromatography system | Thermo Dionex Ultimate 3000 | Agilent 8860 |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) | Agilent J&W DB-35ms (30 m x 0.25 mm, 0.25 µm) |
| MS Type | ESI | EI |
| MS instrument type | Orbitrap | Single quadrupole |
| MS instrument name | Thermo Q Exactive Orbitrap | Agilent 5977C |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | Ion Counts (AUC) | Ion Counts (AUC) |
MS:
| MS ID: | MS004909 |
| Analysis ID: | AN005174 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The MS was operated in negative full scan mode (m/z range: 70–900 and 400-500) using a spray voltage of 4.0 kV, capillary temperature of 350 °C, sheath gas at 50.0, auxiliary gas at 10.0. The AGC target was set at 3.0E6 using a resolution if 140000, with a maximum IT fill time of 512 ms. Data was collected and analyzed using the Xcalibur software (Thermo Scientific) considering a 5 ppm error. The chromatographic peaks for UDP, UTP, cytidine, uridine and glutamine were normalized to the internal standard glutaric acid. Tumor interstitial fluid samples were interpolated to the standard curve and normalized for tumor interstitial fluid volume in order to quantify metabolite concentration. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS004910 |
| Analysis ID: | AN005175 |
| Instrument Name: | Agilent 5977C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | 5977C quipped with EI inert source. Mass spectrometry was performed at 70 eV and a mass range of 150-650 atomic mass units was measured. The chromatographic peaks were extracted from raw chromatograms with a custom MATLAB Script. The chromatographic peaks for 12C-Glucose were normalized to the internal standard 13C6-glucose. Tumor interstitial fluid samples were interpolated to the standard curve and normalized for tumor interstitial fluid volume to quantify metabolite concentration. |
| Ion Mode: | POSITIVE |
