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MB Sample ID: SA342895
| Local Sample ID: | WL_RPLC_POS_WT_2 |
| Subject ID: | SU003281 |
| Subject Type: | Insect |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005187 | AN005188 | AN005189 | AN005190 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish Flex UHPLC Systems | Thermo Vanquish Flex UHPLC Systems | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | HILICON iHILIC-(P) Classic (100 x 2.1mm,5um) | HILICON iHILIC-(P) Classic (100 x 2.1mm,5um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | QTOF | Orbitrap |
| MS instrument name | Thermo Orbitrap ID-X tribrid | Thermo Orbitrap ID-X tribrid | Agilent 6545 QTOF | Thermo Orbitrap ID-X tribrid |
| Ion Mode | NEGATIVE | NEGATIVE | POSITIVE | POSITIVE |
| Units | peak area | peak area | peak area | peak area |
MS:
| MS ID: | MS004920 |
| Analysis ID: | AN005187 |
| Instrument Name: | Thermo Orbitrap ID-X tribrid |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data were collected with the following MS source settings: spray voltage, -2.8 kV; sheath gas, 50; auxiliary gas, 10; sweep gas, 1; ion transfer tube temperature, 300°C; vaporizer temperature, 200°C; mass range, 67 – 1000 Da; resolution, 120,000; maximum injection time, 200 ms; isolation window, 1.6 Da. XCMS and Skyline software were used for data processing |
| Ion Mode: | NEGATIVE |
| MS ID: | MS004921 |
| Analysis ID: | AN005188 |
| Instrument Name: | Thermo Orbitrap ID-X tribrid |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data were collected with the following MS source settings: spray voltage, -2.8 kV; sheath gas, 50; auxiliary gas, 10; sweep gas, 1; ion transfer tube temperature, 300°C; vaporizer temperature, 200°C; mass range, 67 – 1000 Da; resolution, 120,000; maximum injection time, 200 ms; isolation window, 1.6 Da. XCMS and Skyline software were used for data processing |
| Ion Mode: | NEGATIVE |
| MS ID: | MS004922 |
| Analysis ID: | AN005189 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Data were collected with the following MS source settings: gas temp, 250°C; drying gas, 11 l/min; nebulizer, 35 psi; sheath gas temp, 300°C; sheath gas flow 12 l/min; capillary voltage, 3 kV; nozzle voltage, 500 V; isolation window, 1.3 Da. XCMS and Skyline software were used for data processing |
| Ion Mode: | POSITIVE |
| MS ID: | MS004923 |
| Analysis ID: | AN005190 |
| Instrument Name: | Thermo Orbitrap ID-X tribrid |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Data were collected with the following MS source settings: spray voltage, -3 kV; sheath gas, 50; auxiliary gas, 10; sweep gas, 1; ion transfer tube temperature, 300°C; vaporizer temperature, 350°C; mass range, 100 – 1500 Da; resolution, 120,000; maximum injection time, 150 ms; isolation window, 1.6 Da. XCMS and Skyline software were used for data processing |
| Ion Mode: | POSITIVE |
