Return to study ST003173 main page
MB Sample ID: SA343154
| Local Sample ID: | Enzyme assay_Apple pear hybrid_HybASR3 |
| Subject ID: | SU003292 |
| Subject Type: | Plant |
| Subject Species: | Malus domestica/Pyrus communis/Apple-pear intergeneric hybrid |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005207 | AN005208 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Waters Xevo TQ-XS | Waters Xevo TQ-XS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | mg/L | mg/L |
MS:
| MS ID: | MS004940 |
| Analysis ID: | AN005207 |
| Instrument Name: | Waters Xevo TQ-XS |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Capillary voltage was 3.5 kV in positive mode and −2.5 kV in negative mode; the source was kept at 150 °C; desolvation temperature was 500 °C; cone gas flow, 50 L/h; and desolvation gas flow, 800 L/h. Unit resolution was applied to each quadrupole. Flow injections of each individual metabolite were used to optimize the MRM conditions. For the majority of the metabolites, this was done automatically by the Waters Intellistart software, whereas for some compounds the optimal cone voltages and collision energies were identified during collision-induced dissociation (CID) experiments and manually set. A dwell time of at least 25 ms was applied to each MRM transition. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004941 |
| Analysis ID: | AN005208 |
| Instrument Name: | Waters Xevo TQ-XS |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Capillary voltage was 3.5 kV in positive mode and −2.5 kV in negative mode; the source was kept at 150 °C; desolvation temperature was 500 °C; cone gas flow, 50 L/h; and desolvation gas flow, 800 L/h. Unit resolution was applied to each quadrupole. Flow injections of each individual metabolite were used to optimize the MRM conditions. For the majority of the metabolites, this was done automatically by the Waters Intellistart software, whereas for some compounds the optimal cone voltages and collision energies were identified during collision-induced dissociation (CID) experiments and manually set. A dwell time of at least 25 ms was applied to each MRM transition. |
| Ion Mode: | NEGATIVE |
