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MB Sample ID: SA343209
| Local Sample ID: | Mul1(-/-)_TZ 39 |
| Subject ID: | SU003294 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Wild type and Mul1(+/+) mice |
| Age Or Age Range: | 8 weeks |
| Gender: | Male and female |
| Animal Light Cycle: | 12-hour/12-hour light/dark cycle |
| Animal Feed: | Research Diets D12492 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005211 | AN005212 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | A.U. | A.U. |
MS:
| MS ID: | MS004944 |
| Analysis ID: | AN005211 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The aqueous phase was analyzed using the Thermo Q-Exactive Orbitrap mass spectrometer with Dionex UHPLC and autosampler. All samples were analyzed in positive and negative simultaneously with heated electrospray ionization (HESI) with a mass resolution of 35,000 at m/z 200 as separate injections. The polar metabolites were analyzed on Thermo Q-Exactive Orbitrap mass spectrometer equipped with UHPLC. Data were analyzed with MZmine and features were aligned for identification across samples. The metabolites were searched against the Southeastern Center for Integrated Metabolomics (SECIM) metabolite library using retention time and corresponding mass spectral data. Lipids isolated from Folch extraction procedure were also analyzed by the same instrument. Lipidomics data were analyzed using the LipidMatch software and identified lipid entities were exported in a tabular form. |
| Ion Mode: | POSITIVE |
| MS ID: | MS004945 |
| Analysis ID: | AN005212 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The aqueous phase was analyzed using the Thermo Q-Exactive Orbitrap mass spectrometer with Dionex UHPLC and autosampler. All samples were analyzed in positive and negative simultaneously with heated electrospray ionization (HESI) with a mass resolution of 35,000 at m/z 200 as separate injections. The polar metabolites were analyzed on Thermo Q-Exactive Orbitrap mass spectrometer equipped with UHPLC. Data were analyzed with MZmine and features were aligned for identification across samples. The metabolites were searched against the Southeastern Center for Integrated Metabolomics (SECIM) metabolite library using retention time and corresponding mass spectral data. Lipids isolated from Folch extraction procedure were also analyzed by the same instrument. Lipidomics data were analyzed using the LipidMatch software and identified lipid entities were exported in a tabular form. |
| Ion Mode: | NEGATIVE |
