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MB Sample ID: SA351671
| Local Sample ID: | 2738-6 |
| Subject ID: | SU003330 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005266 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Shimadzu GC-2030 |
| Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | EI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Shimadzu TQ8050NX |
| Ion Mode | POSITIVE |
| Units | Relative abundance |
MS:
| MS ID: | MS004997 |
| Analysis ID: | AN005266 |
| Instrument Name: | Shimadzu TQ8050NX |
| Instrument Type: | Triple quadrupole |
| MS Type: | EI |
| MS Comments: | The GC-MS system used comprised of an AOC6000 autosampler, a 2030 Shimadzu gas chromatograph and a TQ8050NX triple quadrupole mass spectrometer (Shimadzu, Japan)with an electron ionisation source(-70eV). The mass spectrometer was tuned according to the manufacturer’s recommendations using tris-(perfluorobutyl)-amine (CF43). GC-MS was performed on a 30m Agilent DB-5 column with 0.25mm internal diameter column and 1µm film thickness. The injection temperature (inlet) was set at 280°C, the MS transfer line at 280°C and the ion source adjusted to 200°C. Helium was used as the carrier gas at a flow rate of 1 mL/min and argon gas was used in the collision cell to generate the MRM product ion. The analysis of the derivatised samples was performed under the following oven temperature program; 100°C start temperature, hold for 4 minutes, followed by a 10°C/min oven temperature ramp to 320°C with a following final hold for 11 minutes. |
| Ion Mode: | POSITIVE |
