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MB Sample ID: SA352099
Local Sample ID: | AWT-Sham_1 |
Subject ID: | SU003341 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005283 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Nexera LC-40 |
Column | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex 6500+ |
Ion Mode | UNSPECIFIED |
Units | lipid content in the sample (nmol/g) |
MS:
MS ID: | MS005013 |
Analysis ID: | AN005283 |
Instrument Name: | ABI Sciex 6500+ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (QTRAP), QTRAP® 6500+ LC-MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (Sciex). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 500 ℃; ion spray voltage (IS) 5500 V(Positive),-4500 V(Neagtive); Ion source gas 1 (GS1), gas 2 (GS2), curtain gas (CUR) were set at 45, 55, and 35 psi, respectively. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to 5 psi. DP and CE for individual MRM transitions was done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the lipids eluted within this period. |
Ion Mode: | UNSPECIFIED |