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MB Sample ID: SA353372

Local Sample ID:40-08-18-2022-POS-Isaac-S16
Subject ID:SU003362
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male and female

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Combined analysis:

Analysis ID AN005313
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Perkin Elmer Brownlee SPP C18 (75 x 2.1mm, 2.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE
Units Normalized to the median value of total lipid signals

MS:

MS ID:MS005043
Analysis ID:AN005313
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:MS2 scan conditions were applied in positive mode, the scan range was from m/z 120–1000, resolution was 120,000 for MS, and 30,000 for DDMS2 (top 10), and the AGC target was 3E6 for full MS and 1E5 for DDMS2, allowing ions to accumulate for up to 200 ms for MS and 50 ms for MS/MS. For MS/MS, the following settings are used: isolation window width 1.2 m/z, stepped NCE at 10, 15, and 25 a.u., minimum AGC 5E2, and dynamic exclusion of previously sampled peaks for 8 s. Quality control (QC) samples were included to check the technical variability and were prepared by mixing an equal volume of lipid extract from each tissue or serum sample. QC samples were included in the analysis sequence every ten samples and monitored for changes in peak area, width, and retention time to determine the performance of the LC-MS/MS analysis. QC samples were subsequently used to align the analytical batches. The lipid peaks were identified, aligned, and exported using MS-DIAL. The data were further normalized to the median value of total lipid signals. Only lipids fully identified by MS2 spectra were included in the analysis.
Ion Mode:POSITIVE
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