Return to study ST003251 main page
MB Sample ID: SA353514
Local Sample ID: | MM_25_0h |
Subject ID: | SU003370 |
Subject Type: | Bacteria |
Subject Species: | Mycoplasma mycoides, Minimal cell JCVI-syn3B |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005325 | AN005326 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | None (Direct infusion) | None (Direct infusion) |
Chromatography system | none | none |
Column | none | none |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | mol percent | mol percent |
MS:
MS ID: | MS005055 |
Analysis ID: | AN005325 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Samples are analyzed by direct infusion on a QExactive mass spectrometer (Thermo Scientific) equipped with a TriVersa NanoMate ion source (Advion Biosciences). Samples are analyzed in both positive and negative ion modes with a resolution of Rm/z=200=280000 for MS (species structural resolution) and Rm/z=200=17500 for MSMS (subspecies structural resolution) experiments, in a single acquisition. MSMS is triggered by an inclusion list encompassing corresponding MS mass ranges scanned in 1 Da increments. Both MS and MSMS data are combined to monitor CE, DAG and TAG ions as ammonium adducts; cholesterol as ammonium adduct of an acetylated derivative. |
Ion Mode: | POSITIVE |
MS ID: | MS005056 |
Analysis ID: | AN005326 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Samples are analyzed by direct infusion on a QExactive mass spectrometer (Thermo Scientific) equipped with a TriVersa NanoMate ion source (Advion Biosciences). Samples are analyzed in both positive and negative ion modes with a resolution of Rm/z=200=280000 for MS (species structural resolution) and Rm/z=200=17500 for MSMS (subspecies structural resolution) experiments, in a single acquisition. MSMS is triggered by an inclusion list encompassing corresponding MS mass ranges scanned in 1 Da increments. Both MS and MSMS data are combined to monitor PC, PC O-, as acetate adducts; and CL, PA, PE, PE O-, PG, PI and PS as deprotonated anions. MS only is used to monitor LPA, LPE, LPE O-, LPI and LPS as deprotonated anions; Cer, HexCer, SM, LPC and LPC O- as acetate adducts. |
Ion Mode: | NEGATIVE |