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MB Sample ID: SA353522
| Local Sample ID: | MM_33.5 (adapted) |
| Subject ID: | SU003370 |
| Subject Type: | Bacteria |
| Subject Species: | Mycoplasma mycoides, Minimal cell JCVI-syn3B |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005325 | AN005326 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | none | none |
| Column | none | none |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | mol percent | mol percent |
MS:
| MS ID: | MS005055 |
| Analysis ID: | AN005325 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Samples are analyzed by direct infusion on a QExactive mass spectrometer (Thermo Scientific) equipped with a TriVersa NanoMate ion source (Advion Biosciences). Samples are analyzed in both positive and negative ion modes with a resolution of Rm/z=200=280000 for MS (species structural resolution) and Rm/z=200=17500 for MSMS (subspecies structural resolution) experiments, in a single acquisition. MSMS is triggered by an inclusion list encompassing corresponding MS mass ranges scanned in 1 Da increments. Both MS and MSMS data are combined to monitor CE, DAG and TAG ions as ammonium adducts; cholesterol as ammonium adduct of an acetylated derivative. |
| Ion Mode: | POSITIVE |
| MS ID: | MS005056 |
| Analysis ID: | AN005326 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Samples are analyzed by direct infusion on a QExactive mass spectrometer (Thermo Scientific) equipped with a TriVersa NanoMate ion source (Advion Biosciences). Samples are analyzed in both positive and negative ion modes with a resolution of Rm/z=200=280000 for MS (species structural resolution) and Rm/z=200=17500 for MSMS (subspecies structural resolution) experiments, in a single acquisition. MSMS is triggered by an inclusion list encompassing corresponding MS mass ranges scanned in 1 Da increments. Both MS and MSMS data are combined to monitor PC, PC O-, as acetate adducts; and CL, PA, PE, PE O-, PG, PI and PS as deprotonated anions. MS only is used to monitor LPA, LPE, LPE O-, LPI and LPS as deprotonated anions; Cer, HexCer, SM, LPC and LPC O- as acetate adducts. |
| Ion Mode: | NEGATIVE |
