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MB Sample ID: SA353977
Local Sample ID: | 210317_MDA_ZEB1_TC_pre_RSL3_WT_n3_24h_RSL3_10uM_48 |
Subject ID: | SU003375 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Combined analysis:
Analysis ID | AN005338 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity H-Class |
Column | Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTRAP |
MS instrument name | ABI Sciex 6500+ |
Ion Mode | NEGATIVE |
Units | absolute intensities |
MS:
MS ID: | MS005068 |
Analysis ID: | AN005338 |
Instrument Name: | ABI Sciex 6500+ |
Instrument Type: | QTRAP |
MS Type: | ESI |
MS Comments: | Targeted MRM with pre-optimized settings and subsequent automated integration of selected signals using Analyst 1.6.3 or Analyst 1.7.1 (Sciex). Oxidized phospholipid species were identified by the fragmentation of [M-H]- (Ox-PE, Ox-PI) or [M+OAc]- (Ox-PC) to the saturated fatty acid anion (16:0 and 18:0) and either the PUFA anion (20:4 and 22:4) with one to three oxygen incorporated or a secondary fragment. Oxidized phospholipids were quantified based on the most intensive, specific transition to the oxidized fatty acid anions. |
Ion Mode: | NEGATIVE |