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MB Sample ID: SA354269
| Local Sample ID: | K3 |
| Subject ID: | SU003385 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005349 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Focus |
| Ion Mode | UNSPECIFIED |
| Units | Peak area |
MS:
| MS ID: | MS005079 |
| Analysis ID: | AN005349 |
| Instrument Name: | Thermo Q Exactive Focus |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The Q Exactive mass spectrometer was operated in full MS and polarity switching mode (acquisition from m/z 50 to 500) with a scan resolution set at 35,000. Five independent cell cultures were measured for each condition and samples were randomised in order to avoid bias in sample analyses due to machine drift. Q Exactive LC-MS/MS vendor raw data files were converted to the open source format mzML using the program ProteoWizard, and then the XCMS program was used for peak alignment, retention time correction and peak area extraction. Significance was determined using an unpaired Student's t-test. p-value < 0.05 was considered as statistically significant. The acquired spectra were analysed using XCalibur Qual Browser and XCalibur Quan Browser software (Thermo Scientific) by referencing to an internal library of compounds. |
| Ion Mode: | UNSPECIFIED |
