Summary of Study ST001298
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000879. The data can be accessed directly via it's Project DOI: 10.21228/M82M47 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001298 |
Study Title | Metabolome Profiling of Synechococcus elongatus PCC 11801 strains engineered for Succinate Production |
Study Type | Measurement of relative metabolite pools of wild type and engineered Synechococcus elongatus PCC 11801 strains using Isotopic Ratio Method |
Study Summary | Experiments to measure relative metabolite pools of wild type Synechococcus elongatus PCC 11801 and its recombinants producing succinate. The wild type and the engineered strains producing succinate were cultivated at 1% CO2 and their metabolome data was collected in three biological and three technical replicates (n=9). The study aims to find metabolomics changes between the wild type and the engineered to identify potential rate-limiting steps that be used as targets for improved production. |
Institute | Indian Institute of Technology Bombay |
Department | Department of Chemical Engineering |
Laboratory | Bio systems Engineering Lab |
Last Name | Wangikar |
First Name | Pramod P |
Address | Department of Chemical Engineering, IIT Bombay, Powai, Mumbai, Maharashtra, India - 400076 |
wangikar@iitb.ac.in | |
Phone | 22254215 |
Submit Date | 2020-01-03 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2020-07-05 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN002162 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Shimadzu 20AD |
Column | Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | ABI Sciex 5600+ TripleTOF |
Ion Mode | NEGATIVE |
Units | Area Ratio |
MS:
MS ID: | MS002011 |
Analysis ID: | AN002162 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | The peak areas corresponding to the 12C and 13C monoisotopic peak for the metabolites of interest were quantified using MultiQuant 3.0.1 (SCIEX, Framingham, MA). The relative quantification of metabolites was done using isotopic ratio method by normalizing area under the peak for monoisotopic m/z of a particular metabolite by its respective highest possible isotopologue present in the internal standard giving area ratio. |
Ion Mode: | NEGATIVE |