Summary of Study ST001393
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000956. The data can be accessed directly via it's Project DOI: 10.21228/M84386 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001393 |
Study Title | Sea-ice diatom compatible solute shifts |
Study Type | Compatible solutes were quantified in sea-ice diatoms |
Study Summary | Sea-ice algae provide an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures of the Antarctic sea-ice diatom, Nitzschia lecointei, displayed large differences in their metabolomes when grown in a matrix of conditions that included temperatures of –1 and 4°C, and salinities of 32 and 41, despite relatively small changes in growth rate. Temperature exerted a greater effect than salinity on cellular metabolite pool sizes, though the N- or S-containing compatible solutes, 2,3-dihydroxypropane-1-sulfonate (DHPS), glycine betaine (GBT), dimethylsulfoniopropionate (DMSP), and proline responded strongly to both temperature and salinity, suggesting complexity in their control. We saw the largest (> 4 fold) response to salinity for proline. DHPS, a rarely studied but potential compatible solute, reached the highest intracellular compatible solute concentrations of ~ 85 mM. When comparing the culture findings to natural Arctic sea-ice diatom communities, we found extensive overlap in metabolite profiles, highlighting the relevance of culture-based studies to probe environmental questions. Large changes in sea-ice diatom metabolomes and compatible solutes over a seasonal cycle could be significant components of biogeochemical cycling within sea ice. |
Institute | University of Washington |
Department | School of Oceanography |
Laboratory | Ingalls Lab |
Last Name | Dawson |
First Name | Hannah |
Address | 1501 NE Boat Street, Marine Science Building, Room G, Seattle, WA 98195 |
hmdawson@uw.edu | |
Phone | 2062216750 |
Submit Date | 2020-03-24 |
Publications | Dawson et al., Elementa |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2020-09-29 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002323 | AN002324 | AN002325 | AN002326 | AN002327 | AN002328 | AN002329 | AN002330 | AN002331 |
---|---|---|---|---|---|---|---|---|---|
Analysis type | MS | MS | MS | MS | MS | MS | MS | MS | MS |
Chromatography type | HILIC | HILIC | HILIC | HILIC | HILIC | HILIC | HILIC | HILIC | Reversed phase |
Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class |
Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | Waters Acquity UPLC HSS Cyano (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI | ESI | ESI | ESI | ESI | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole | Orbitrap | Orbitrap | Orbitrap | Orbitrap | Triple quadrupole |
MS instrument name | Waters Xevo-TQ-S | Waters Xevo-TQ-S | Waters Xevo-TQ-S | Waters Xevo-TQ-S | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Waters Xevo-TQ-S |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE | POSITIVE |
Units | Normalized Peak Area Per L Seawater | Normalized Peak Area Per RFU | Normalized Peak Area Per L Seawater | Normalized Peak Area Per RFU | Normalized Peak Area Per L Seawater | Normalized Peak Area Per RFU | Normalized Peak Area Per L Seawater | Normalized Peak Area Per RFU | Normalized Peak Area Per RFU |
MS:
MS ID: | MS002165 |
Analysis ID: | AN002323 |
Instrument Name: | Waters Xevo-TQ-S |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | See protocol, data from culture samples |
Ion Mode: | POSITIVE |
MS ID: | MS002166 |
Analysis ID: | AN002324 |
Instrument Name: | Waters Xevo-TQ-S |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | See protocol, data from field samples |
Ion Mode: | POSITIVE |
MS ID: | MS002167 |
Analysis ID: | AN002325 |
Instrument Name: | Waters Xevo-TQ-S |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | See protocol, data from culture samples |
Ion Mode: | NEGATIVE |
MS ID: | MS002168 |
Analysis ID: | AN002326 |
Instrument Name: | Waters Xevo-TQ-S |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | See protocol, data from field samples |
Ion Mode: | NEGATIVE |
MS ID: | MS002169 |
Analysis ID: | AN002327 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | See protocol, data from culture samples |
Ion Mode: | POSITIVE |
MS ID: | MS002170 |
Analysis ID: | AN002328 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | See protocol, data from field samples |
Ion Mode: | POSITIVE |
MS ID: | MS002171 |
Analysis ID: | AN002329 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | See protocol, data from culture samples |
Ion Mode: | NEGATIVE |
MS ID: | MS002172 |
Analysis ID: | AN002330 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | See protocol, data from field samples |
Ion Mode: | NEGATIVE |
MS ID: | MS002173 |
Analysis ID: | AN002331 |
Instrument Name: | Waters Xevo-TQ-S |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | See protocol, data from culture samples |
Ion Mode: | POSITIVE |