Summary of Study ST001822
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001151. The data can be accessed directly via it's Project DOI: 10.21228/M8XH6Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001822 |
Study Title | The RNA-binding protein RBP42 regulates cellular energy metabolism in mammalian-infective Trypanosoma brucei |
Study Summary | Metabolic changes following two days of RBP42 knockdown was investigated using a targeted metabolomics approach, designed to capture intermediary metabolites in central carbon metabolism including glycolytic intermediates, TCA compounds, amino acids, nucleotides and derivatives, were obtained using hydrophilic interaction liquid chromatography (HILIC) separation method coupled with mass spectrometry run in negative ionization mode |
Institute | Rutgers University |
Last Name | Das |
First Name | Anish |
Address | Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers-New Jersey Medical School, Newark, NJ 07103 |
dasak@njms.rutgers.edu | |
Phone | 9739723978 |
Submit Date | 2021-06-07 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2021-06-10 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002958 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap |
Ion Mode | NEGATIVE |
Units | arbitrary |
MS:
MS ID: | MS002748 |
Analysis ID: | AN002958 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Each sample was analyzed with a nominal resolution of 70,000. The automatic gain control target was 3×106. The maximum injection time was 50 ms. Scan range was 75-1,000. The targeted metabolite data analysis was performed in MAVEN. The compound identification was based on the accurate mass and the retention time learned from in-house chemical collection |
Ion Mode: | NEGATIVE |