Summary of Study ST001935
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001224. The data can be accessed directly via it's Project DOI: 10.21228/M8H12N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001935 |
Study Title | Metabolomic profiling of spontaneous macaque model for diabetes mellitus |
Study Summary | The prevalence of diabetes mellitus has been increasing for decades worldwide. To develop safe and potent therapeutics, insights into the mechanisms underlying its pathogenesis are urgently needed. We reported the multi-omics profiling of the liver and sera of both peripheral blood and hepatic portal vein blood from Macaca fascicularis with spontaneous diabetes mellitus with a chow diet. The other two groups of the monkeys fed with chow diet and high-sugar high-fat (HSHF) diet, respectively, were included for comparison. These multi-omics datasets can provide a comprehensive picture of the molecular changes caused by diabetes in primates. Analyses of various omics datasets revealed the alterations of high consistency. Correlation between transcripts and proteins derived from the same genes was observed across individuals for most genes, especially the ones of differential expression. As a result, we found that distinct patterns of the metabolome, proteome, and transcriptome between spontaneous diabetes and HSHF diet-induced diabetes compared with healthy individuals. |
Institute | Xiamen University |
Last Name | Yang |
First Name | Zhu |
Address | OEW901, Oen Hall Building West Wing,, Ho Sin Hang Campus, Hong Kong Baptist University |
yangzhu@gmail.com | |
Phone | (+852)34115162 |
Submit Date | 2021-09-18 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | GC-MS |
Release Date | 2022-02-19 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003145 | AN003146 | AN003147 | AN003148 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Shim-pack UFLC SHIMADZU CBM A system | Shim-pack UFLC SHIMADZU CBM A system | Shim-pack UFLC SHIMADZU CBM A system | Shim-pack UFLC SHIMADZU CBM A system |
Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
MS Type | EI | EI | EI | EI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | Peak area | Peak area | Peak area | Peak area |
MS:
MS ID: | MS002925 |
Analysis ID: | AN003145 |
Instrument Name: | ABI Sciex 6500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | EI |
MS Comments: | LIT and triple quadrupole (QQQ) scans were acquired in both positive and negative ion modes under the control of Analyst 1.6.3 software (Sciex, Washington, USA). The ion spray voltage (IS) of the ESI source was 5500 and -4500 V for positive and negative modes, respectively. Source temperature was set at 500 C, ion source gas I (GSI), gas II (GSII), curtain gas (CUR) at 55, 60, and 25.0 psi, respectively, and collision gas (CAD) high. Instrument tuning and mass calibration in QQQ and LIT modes were performed with 10 and 100 μmol/L polypropylene glycol solutions, respectively. Specific sets of multiple reaction monitoring (MRM) transitions of various periods of retention time were monitored according to an in-house library of metabolites. |
Ion Mode: | POSITIVE |
MS ID: | MS002926 |
Analysis ID: | AN003146 |
Instrument Name: | ABI Sciex 6500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | EI |
MS Comments: | LIT and triple quadrupole (QQQ) scans were acquired in both positive and negative ion modes under the control of Analyst 1.6.3 software (Sciex, Washington, USA). The ion spray voltage (IS) of the ESI source was 5500 and -4500 V for positive and negative modes, respectively. Source temperature was set at 500 C, ion source gas I (GSI), gas II (GSII), curtain gas (CUR) at 55, 60, and 25.0 psi, respectively, and collision gas (CAD) high. Instrument tuning and mass calibration in QQQ and LIT modes were performed with 10 and 100 μmol/L polypropylene glycol solutions, respectively. Specific sets of multiple reaction monitoring (MRM) transitions of various periods of retention time were monitored according to an in-house library of metabolites. |
Ion Mode: | NEGATIVE |
MS ID: | MS002927 |
Analysis ID: | AN003147 |
Instrument Name: | ABI Sciex 6500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | EI |
MS Comments: | LIT and triple quadrupole (QQQ) scans were acquired in both positive and negative ion modes under the control of Analyst 1.6.3 software (Sciex, Washington, USA). The ion spray voltage (IS) of the ESI source was 5500 and -4500 V for positive and negative modes, respectively. Source temperature was set at 500 C, ion source gas I (GSI), gas II (GSII), curtain gas (CUR) at 55, 60, and 25.0 psi, respectively, and collision gas (CAD) high. Instrument tuning and mass calibration in QQQ and LIT modes were performed with 10 and 100 μmol/L polypropylene glycol solutions, respectively. Specific sets of multiple reaction monitoring (MRM) transitions of various periods of retention time were monitored according to an in-house library of metabolites. |
Ion Mode: | POSITIVE |
MS ID: | MS002928 |
Analysis ID: | AN003148 |
Instrument Name: | ABI Sciex 6500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | EI |
MS Comments: | LIT and triple quadrupole (QQQ) scans were acquired in both positive and negative ion modes under the control of Analyst 1.6.3 software (Sciex, Washington, USA). The ion spray voltage (IS) of the ESI source was 5500 and -4500 V for positive and negative modes, respectively. Source temperature was set at 500 C, ion source gas I (GSI), gas II (GSII), curtain gas (CUR) at 55, 60, and 25.0 psi, respectively, and collision gas (CAD) high. Instrument tuning and mass calibration in QQQ and LIT modes were performed with 10 and 100 μmol/L polypropylene glycol solutions, respectively. Specific sets of multiple reaction monitoring (MRM) transitions of various periods of retention time were monitored according to an in-house library of metabolites. |
Ion Mode: | NEGATIVE |