Summary of Study ST001992
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001265. The data can be accessed directly via it's Project DOI: 10.21228/M86D99 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001992 |
Study Title | Dynamics of bile acid metabolism between the host and gut microbiome in progression to islet autoimmunity (Feces) |
Study Summary | Previous studies suggest that the human gut microbiome is dysregulated in islet autoimmunity, preceding the clinical onset of type 1 diabetes (T1D). The Gut microbiota of the gut plays an important role in the regulation of bile acid (BA) metabolism. However, not much is known about the regulation of BAs during progression to T1D. Here, we analyzed BAs in a longitudinal series of serum (n= 333) and stool (n= 304) samples, collected at 3, 6, 12, 18, 24 and 36 months of age, from children who developed a single islet autoantibody (P1Ab), multiple islet autoantibodies (P2Ab), and controls (CTRs) who remained autoantibody (AAb) negative during the follow-up. In addition, we analyzed the stool microbiome by shotgun metagenomics in a subgroup of these children (n=111). Factor analysis showed that age had the strongest impact on BA and microbiome profiles. We found that, at an early age, the systemic BA (including taurine and glycine conjugates) and microbial secondary BA pathways were altered in the P2Ab group as compared to the P1Ab or CTR groups. Our findings thus suggest that dysregulated BA metabolism in early life may contribute to the risk and pathogenesis of T1D. |
Institute | University of Turku |
Department | University of Turku |
Laboratory | Turku Metabolomics Center |
Last Name | Lamichhane |
First Name | Santosh |
Address | Yo Kylä 30A 6 |
santosh.lamichhane@utu.fi | |
Phone | 0452299070 |
Submit Date | 2021-11-15 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-01-31 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003249 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters |
Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap |
Ion Mode | NEGATIVE |
Units | ng/ml |
MS:
MS ID: | MS003022 |
Analysis ID: | AN003249 |
Instrument Name: | ABI Sciex 5500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | The bile acids were measured in fecal sample as described based on . 20 μL of serum, using the same internal standard mixtures, was filtered through a frit filter plate (96-Well Protein Precipitation Filter Plate, Sigma Aldrich), and the effluent was collected and evaporated to dryness and the residue was dissolved in 20 μL of a 40:60 MeOH: H2O v/v mixture containing the same injection standards. Analyses were performed on an ACQUITY UPLC system coupled to a triple quadrupole mass spectrometer (Waters Corporation, Milford, USA) with an atmospheric electrospray interface operating in negative ion mode. An external calibration with six calibration points (0.5–600 ng/mL), including a solvent blank, was carried out for use in quantitation. |
Ion Mode: | NEGATIVE |