Summary of Study ST001995
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001267. The data can be accessed directly via it's Project DOI: 10.21228/M8XX3Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001995 |
Study Title | Mutasynthetic production and antimicrobial characterisation of Darobactin darobactin analogs (MS analysis) |
Study Summary | There is great need for therapeutics against multi-drug resistant, Gram-negative bacterial pathogens. Recently, darobactin A, a novel bicyclic heptapeptide that selectively kills Gram-negative bacteria by targeting the outer-membrane protein BamA, was discovered. Its efficacy was proven in animal infection models of Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa, thus promoting darobactin A as a promising lead compound. Originally discovered from members of the nematode symbiotic genus Photorhabdus, the biosynthetic gene cluster (BGC) encoding for the synthesis of darobactin A can also be found in other γ-proteobacterial families. Therein, the precursor peptides DarB-F, which differ in their core sequence from darobactin A, were identified in silico. Even though production of these analogs was not observed in the putative producer strains, we were able to generate them by mutasynthetic derivatization of a heterologous expression system. The generated analogs were isolated and tested for their bioactivity. The most potent compound, darobactin B, was used for co-crystallization with the target BamA, revealing an identical binding site to darobactin A. Besides its potency, darobactin B did not exhibit cytotoxicity and was slightly more active against Acinetobacter baumanii isolates than darobactin A. Furthermore, we evaluated the plasma protein binding of darobactin A and B, indicating their different pharmacokinetic properties. This is the first report on new members of this new antibiotics class, which is likely to expand to several promising therapeutic candidates. |
Institute | Justus-Liebig-University Giessen |
Laboratory | Schäberle Laboratory |
Last Name | Mettal |
First Name | Ute |
Address | Ohlebergsweg 12, Gießen, Hesse, 35392, Germany |
Ute.Mettal@chemie.uni-giessen.de | |
Phone | +49 641 97219 142 |
Submit Date | 2021-11-18 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML, d |
Analysis Type Detail | LC-MS |
Release Date | 2022-11-21 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003252 | AN003253 | AN003254 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Agilent 1290 Infinity | Agilent 1290 Infinity |
Column | Macherey-Nagel EC 100/2 Nucleoshell C18 2.7μm | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
MS Type | ESI | ESI | ESI |
MS instrument type | QTOF | QTOF | QTOF |
MS instrument name | Bruker micrOTOFQ II | Bruker micrOTOFQ II | Bruker maXis II |
Ion Mode | POSITIVE | POSITIVE | POSITIVE |
Units | N/A (structure elucidation) | N/A (structure elucidation) | N/A (structure elucidation) |
MS:
MS ID: | MS003024 |
Analysis ID: | AN003252 |
Instrument Name: | Bruker micrOTOFQ II |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | For standard measurements on the Dionex Ultimate 3000 + Bruker micrOTOFQ II system MS data was acquired over a range from 50 to 2000 m/z in positive mode. Auto MS/MS fragmentation was achieved with rising collision energy (for single charged ions: 18–45 eV over a gradient from 100 to 1000 m/z; for double charged ions: 15–32 eV over a gradient from 100 to 1000 m/z). Calibration of mass spectra was achieved using 10 mM sodium formate in H2O/ iPrOH (1:1) as internal standard. Mass spectra were analysed using Bruker Data Analysis 4.2 software (Bruker Daltonics, Bremen, Germany). Masses of the expected compounds were determined using ChemDraw Professional 16.0.1.4 (PerkinElmer, Waltham, USA) and recorded chromatograms were extracted for the respective m/z. Ions with fitting m/z (Δ<10 ppm) and plausible retention time were fragmented and fragments were matched to in silico identified plausible decay products. |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3500 |
Analysis Protocol File: | Mass_Spectrometry_Protocol.docx |
MS ID: | MS003025 |
Analysis ID: | AN003253 |
Instrument Name: | Bruker micrOTOFQ II |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | For standard measurements on the Agilent Infinity 1290 + Bruker micrOTOFQ II system MS data was acquired over a range from 100 to 1500 m/z in positive mode. Auto MS/MS fragmentation was achieved with rising collision energy (for single charged ions: 18–45 eV over a gradient from 100 to 1000 m/z; for double charged ions: 15–32 eV over a gradient from 100 to 1000 m/z). Calibration of mass spectra was achieved using 10 mM sodium formate in H2O/ iPrOH (1:1) as internal standard. Mass spectra were analysed using Bruker Data Analysis 4.2 software (Bruker Daltonics, Bremen, Germany). Masses of the expected compounds were determined using ChemDraw Professional 16.0.1.4 (PerkinElmer, Waltham, USA) and recorded chromatograms were extracted for the respective m/z. Ions with fitting m/z (Δ<10 ppm) and plausible retention time were fragmented and fragments were matched to in silico identified plausible decay products. |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3500 |
Analysis Protocol File: | Mass_Spectrometry_Protocol.docx |
MS ID: | MS003026 |
Analysis ID: | AN003254 |
Instrument Name: | Bruker maXis II |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | For standard measurements on the Agilent Infinity 1290 + Bruker maXis II system MS data was acquired over a range from 50 to 2000 m/z in positive mode. Auto MS/MS fragmentation was achieved with rising collision energy (for single charged ions: 35–70 eV over a gradient from 500 to 2000 m/z; for double charged ions: 25–50 eV over a gradient from 500 to 2000 m/z). Calibration of mass spectra was achieved using sodium formate in H2O/ iPrOH (1:1) as internal standard. Mass spectra were analysed using Bruker Data Analysis 4.2 software (Bruker Daltonics, Bremen, Germany). Masses of the expected compounds were determined using ChemDraw Professional 16.0.1.4 (PerkinElmer, Waltham, USA) and recorded chromatograms were extracted for the respective m/z. Ions with fitting m/z (Δ<10 ppm) and plausible retention time were fragmented and fragments were matched to in silico identified plausible decay products. |
Ion Mode: | POSITIVE |
Capillary Voltage: | 4500 |
Analysis Protocol File: | Mass_Spectrometry_Protocol.docx |