Summary of Study ST002009
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001272. The data can be accessed directly via it's Project DOI: 10.21228/M89402 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002009 |
Study Title | Metabolomics analysis of stress erythroid progenitors |
Study Summary | A time course study to assess the intracellular metabolic changes of progenitors isolated from stress erythropoiesis expansion culture. |
Institute | Pennsylvania State University |
Department | Veterinary and Biomedical Sciences |
Laboratory | Paulson Lab |
Last Name | Paulson |
First Name | Robert |
Address | 228 AVBS Building Shortlidge Road University Park, PA 16802 |
rfp5@psu.edu | |
Phone | 814-863-6306 |
Submit Date | 2021-12-05 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2022-03-11 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003275 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Waters SSelect HSS C18 |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Exactive Plus Orbitrap |
Ion Mode | NEGATIVE |
Units | Normalized peak area |
MS:
MS ID: | MS003047 |
Analysis ID: | AN003275 |
Instrument Name: | Thermo Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Raw data files were converted to .mzML file format using the ProteoWizard software, and they were analyzed by the MS-DIAL software. Metabolites were identified by comparison to an in-house reference library of pure metabolite standards which included mass-to-charge ratio (m/z) and retention time. For quantification of metabolite abundance, peak areas of identified metabolites were first normalized to the internal standard chlorpropamide, and then normalized to cell numbers from each sample. Data were analyzed using R and Cytoscape software. |
Ion Mode: | NEGATIVE |