Summary of Study ST002061
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001305. The data can be accessed directly via it's Project DOI: 10.21228/M81H6N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002061 |
Study Title | Glutamine flux in macrophages treated with stable-isotope labeled analog 4 mM (U-13C5) glutamine |
Study Type | Glutamine flux |
Study Summary | 107 BMDMs per group (Sirt3 WT and Sirt3 K223R)were seeded in 10cm plates and incubated in RPMI-1640 cell culture medium with 10% FBS. Prior to isotopic labeling, the medium was replaced with RPMI-1640 without glutamine for 4 hrs. Then stable-isotope labeled analog 4 mM (U-13C5) glutamine (Cambridge Isotope) was added together with IL-4 (20ng/ml) for 4 hrs. |
Institute | Shanghai Jiao Tong University affiliated Renji Hospital |
Department | Department of Urology |
Laboratory | Cheng Jinke's Lab |
Last Name | Zhou |
First Name | Wei |
Address | N0.280 South Chongqing Road |
joesphchou@alumni.sjtu.edu.cn | |
Phone | +8615216716293 |
Submit Date | 2022-01-19 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2022-02-07 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003360 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | ThermoFisher Ultimate 3000 UHPLC system |
Column | Waters BEH Amide |
MS Type | ESI |
MS instrument type | Hybrid Quadrupole-Orbitrap™ |
MS instrument name | ThermoFisher Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometry (QE) |
Ion Mode | NEGATIVE |
Units | pmoles/I |
MS:
MS ID: | MS003129 |
Analysis ID: | AN003360 |
Instrument Name: | ThermoFisher Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometry (QE) |
Instrument Type: | Hybrid Quadrupole-Orbitrap™ |
MS Type: | ESI |
MS Comments: | The eluents were analyzed on a ThermoFisher Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometry (QE) in Heated Electrospray Ionization Negative (HESI-) mode, separately. Spray voltage was set to 4000 V. Capillary and Probe Heater Temperature were separately 320 °C and 320 °C. Sheath gas flow rate was 35 (Arb, arbitrary unit), and Aux gas flow rate was 10 (Arb). S-Lens RF Level was 50 (Arb). The full scan was operated at a high-resolution of 70000 FWHM (m/z=200) at a range of 70- 1050 m/z with AGC Target setting at 3×106. |
Ion Mode: | NEGATIVE |