Summary of Study ST002231
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001421. The data can be accessed directly via it's Project DOI: 10.21228/M81X41 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002231 |
Study Title | Metabolomics Analysis of HOG-EV and HOG-R132H Cells with and without BAY 2402234 Treatment |
Study Type | Metabolomics Analysis |
Study Summary | HOG cells were plated in 6-well plates (0.5 × 10^6 cells per well). 24 hours later, HOG-EV or HOG-R132H cells were treated for 24 hours with 10 nM BAY 2402234 or DMSO. Cells were then harvested for LC-MS analysis. |
Institute | UT Southwestern Medical Center |
Department | Children's Research Institute |
Laboratory | McBrayer Laboratory |
Last Name | McBrayer |
First Name | Samuel |
Address | 6000 Harry Hines Boulevard, NL10.110K, Dallas, TX 75235, USA |
samuel.mcbrayer@utsouthwestern.edu | |
Phone | (214)-648-3730 |
Submit Date | 2022-07-14 |
Num Groups | 4 |
Total Subjects | 1 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2022-07-28 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003640 | AN003641 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Vanquish Flex UHPLC | Vanquish Flex UHPLC |
Column | Millipore ZIC-pHILIC | Millipore ZIC-pHILIC |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | TIC-Corrected Peak Area | TIC-Corrected Peak Area |
MS:
MS ID: | MS003391 |
Analysis ID: | AN003640 |
Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Spectra were acquired with a resolving power of 60,000 full width at half maximum (FWHM), a scan range set to 80–1,200 m/z, and polarity switching. Data-dependent MS/MS data was acquired on unlabeled pooled samples to confirm metabolite IDs when necessary. Peaks were integrated using El-Maven 0.12.0 software (Elucidata) or TraceFinder 5.1 SP2 software (Thermo Fisher). Total ion counts were quantified using Freestyle 1.7 SP1 software (Thermo Fisher). Peaks were normalized to total ion counts using the R statistical programming language. |
Ion Mode: | POSITIVE |
MS ID: | MS003392 |
Analysis ID: | AN003641 |
Instrument Name: | Thermo Q Exactive HF-X Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Spectra were acquired with a resolving power of 60,000 full width at half maximum (FWHM), a scan range set to 80–1,200 m/z, and polarity switching. Data-dependent MS/MS data was acquired on unlabeled pooled samples to confirm metabolite IDs when necessary. Peaks were integrated using El-Maven 0.12.0 software (Elucidata) or TraceFinder 5.1 SP2 software (Thermo Fisher). Total ion counts were quantified using Freestyle 1.7 SP1 software (Thermo Fisher). Peaks were normalized to total ion counts using the R statistical programming language. |
Ion Mode: | NEGATIVE |