Summary of Study ST002266
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001448. The data can be accessed directly via it's Project DOI: 10.21228/M8JQ4M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002266 |
Study Title | Kīlauea lava fuels phytoplankton bloom in the North Pacific Ocean - study of particulate metabolites |
Study Type | Study of particulate metabolites in phytoplankton blooms. |
Study Summary | These data are relative concentrations of targeted metabolites measured in particulate matter collected from near the Island of Hawai’i in July 2018 during the Kilauea eruption. Six stations were sampled, with station 2 representing the most geothermally impacted station closest to the lava entry. Station 6 was the most oligotrophic station. The particulate metabolites show evidence of altered community composition and metabolism at the geothermally impacted station. This station was within a phytoplankton bloom. The bloom was stimulated by lava heating deep seawater and driving upwelling, which then provided nutrients for diatom growth. See Wilson and Hawco, et. al. 2019 (DOI: 10.1126/science.aax4767) for a complete description of sample collection, phytoplankton bloom dynamics, and chemical modifications of seawater due to the eruption. Metabolites with high concentration (relative abundance per L of seawater) in the geothermally impacted station (St 2) compared to the other stations were: cytosine, hydroxyectoine, adenine, adenosine, thymine, glutamic acid, ectoine, deoxyadenosine, UDP-glucosamine, and guanosine. After normalizing to the particulate carbon concentration at each station, guanosine, glutamic acid, hydroxyectoine, ectoine, adenosine, deoxyadenosine, and UDP-glucosamine were enriched in the geothermally impacted station relative to other stations. Ectoine was the metabolite with the largest change between St 2 and St 6, regardless of normalization to L of seawater or to moles of particulate carbon, with dramatically higher concentrations in the geothermally impacted waters. Except for glutamic and proline, particulate amino acids were generally in higher concentrations in the oligotrophic station. |
Institute | University of Washington |
Department | School of Oceanography |
Laboratory | Ingalls Lab |
Last Name | Lionheart |
First Name | Regina |
Address | 1400 NE Campus Parkway, Seattle, Washington, 98195, USA |
regina16@uw.edu | |
Phone | 2062216750 |
Submit Date | 2022-08-10 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2022-09-05 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003701 | AN003702 | AN003703 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | HILIC | HILIC | GC |
Chromatography system | Q Exactive™ Plus Hybrid Quadrupole-Orbitrap | Q Exactive™ Plus Hybrid Quadrupole-Orbitrap | Q Exactive™ Plus Hybrid Quadrupole-Orbitrap |
Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | Waters Acquity UPLC HSS Cyano (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
Units | Normalized peak area | Normalized peak area | Normalized peak area |
MS:
MS ID: | MS003451 |
Analysis ID: | AN003701 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | See protocol |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Ingalls_Metabolomics_MS_HOT-LAVA.txt |
MS ID: | MS003452 |
Analysis ID: | AN003702 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | See protocol |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Ingalls_Metabolomics_MS_HOT-LAVA.txt |
MS ID: | MS003453 |
Analysis ID: | AN003703 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | See protocol |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Ingalls_Metabolomics_MS_HOT-LAVA.txt |