Summary of Study ST002515
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001620. The data can be accessed directly via it's Project DOI: 10.21228/M89B04 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002515 |
Study Title | Time course 2: Acetate quantification from growth of Eggerthella lenta in defined media |
Study Type | Untargeted LC-MS |
Study Summary | This dataset contains targeted metabolomics analysis of carboxylic acids in supernatants from 3 strains of Eggerthella lenta grown in defined EDM1 media with varying acetate concentrations. |
Institute | University of California, San Francisco |
Last Name | Noecker |
First Name | Cecilia |
Address | 513 Parnassus Ave HSW1501, San Francisco, CA 94143 |
cecilia.noecker@ucsf.edu | |
Phone | 415-502-3264 |
Submit Date | 2023-03-21 |
Raw Data Available | Yes |
Raw Data File Type(s) | d, mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-04-10 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004142 |
---|---|
Analysis type | MS |
Chromatography type | Unspecified |
Chromatography system | Agilent 1290 Infinity II |
Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6545 QTOF |
Ion Mode | NEGATIVE |
Units | mM |
MS:
MS ID: | MS003889 |
Analysis ID: | AN004142 |
Instrument Name: | Agilent 6545 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Samples were injected via refrigerated autosampler into mobile phase and chromatographically separated by an Agilent 1290 Infinity II UPLC and detected using an Agilent 6545XT Q-TOF (quadrupole time of flight) mass spectrometer equipped with a dual jet stream electrospray ionization source, operating under extended dynamic range (1,700 m/z). MS1 spectra were collected in centroid mode, and peak assignments in samples were made based on comparisons of retention times and accurate masses from authentic standards using MassHunter Quantitative Analysis v.10.0 software from Agilent Technologies. Acetate was quantified from calibration curves constructed with acetate-d4 as a standard using isotope-dilution MS with phenylpropionate-d9 as the internal standard. Calibration curves were performed in a modified base form of EDM1 lacking amino acids and other carboxylic acids. A background level of 1.05mM of acetate was subtracted to obtain the final quantities. |
Ion Mode: | NEGATIVE |