Summary of Study ST002786
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001736. The data can be accessed directly via it's Project DOI: 10.21228/M89Q7K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002786 |
Study Title | Metabolic changes of the pig kidney during isolated normothermic perfusion with autologous whole blood - urine |
Study Type | Experimental |
Study Summary | This study investigates how glucose, lactate and 20 amino acids in the urine of normothermically perfused pig kidneys changes over time. It also studies how type and severity of ischemia before perfusion (cold or warm ischemia) change this profile. |
Institute | KU Leuven |
Department | Microbiology, Immunology and Transplantation |
Laboratory | Lab of Abdominal Transplantation |
Last Name | Jochmans |
First Name | Ina |
Address | Herestraat 49, Leuven, 3000, Belgium |
ina.jochmans@kuleuven.be | |
Phone | None |
Submit Date | 2023-07-17 |
Num Groups | 2 |
Total Subjects | 12 |
Num Males | 6 male pigs |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2023-08-07 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004533 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Waters ACQUITY UPLC BEH C18 (150 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Focus |
Ion Mode | NEGATIVE |
Units | Peak area |
MS:
MS ID: | MS004280 |
Analysis ID: | AN004533 |
Instrument Name: | Thermo Q Exactive Focus |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Raw MS data were converted into mzML using the MSConvert tool of Proteowizard (version 3.0.20247). Peak picking was performed with El_Maven (El_Maven, Elucidata, Massachusetts, USA, version 0.12.0). Metabolites were identified using an in-house library containing exact mass and retention time. The mass accuracy during data processing in El Maven was set at 10 ppm. Calculation of abundances was done in the LC-MS Workflow of El_Maven. Raw abundances (peak area values) for each metabolite were corrected for internal standard (Myristic acid d27). These were corrected for urine volume. |
Ion Mode: | NEGATIVE |