Summary of Study ST002923
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001816. The data can be accessed directly via it's Project DOI: 10.21228/M8ZX5S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002923 |
Study Title | Gut sphingolipid metabolites in infants with atopic dermatitis associated with food allergy - Part 1 |
Study Summary | Food allergy (FA) may be present in the range of 20–80% in atopic dermatitis (AD). Food sensitization through the skin can cause FA due to damage to the skin barrier, and failure to acquire tolerance to food allergens in the gut can equally cause the development of FA. Gut metabolites can influence the physical gut barrier and intestinal homeostasis. Therefore, it is possible that gut metabolites related to gut immunity play an important role in the development of FA. Sphingolipids are key factors in cell inflammatory response and affect gut epithelial cells and skin barrier integrity and function. FA is associated with a marked decrease in serum sphingolipid levels. However, there are no reports of FA-associated gut sphingolipid metabolites in infants by targeted metabolomics. In our previous study, we showed that when FA is present in various phenotypes of AD in early life, it might be associated with the later development of asthma. The discovery of a biomarker that can distinguish the phenotypes that accompany AD and FA from other AD phenotypes is therefore expedient. Consequently, we aimed to investigate whether FA in AD infants. can be classified as gut sphingolipid metabolites using targeted metabolomics. |
Institute | Asan Medical Center |
Last Name | Yoo |
First Name | Hyun Ju |
Address | 88, Olympic-ro 43-gil, Songpa-gu |
d131108@ulsan.ac.kr | |
Phone | 0230104029 |
Submit Date | 2023-10-06 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2023-11-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004793 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | Phenomenex Jupiter C4 (50 × 1.0 mm, 5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo LTQ XL |
Ion Mode | NEGATIVE |
Units | pmol/mg |
MS:
MS ID: | MS004539 |
Analysis ID: | AN004793 |
Instrument Name: | Thermo LTQ XL |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Selected ion monitoring (SRM) was uesd in the negative ion mode and the extracted ion chromatogram corresponding to the specific transition (phosphate ion, PO3-, m/z 78.9585) for S1P was used for quantification. Calibration range was 0.01 – 10 μM with R2 > 0.99. Data analysis was performed by using Xcalibur 2.2 software. Ultimate3000 |
Ion Mode: | NEGATIVE |