Summary of Study ST003220
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002008. The data can be accessed directly via it's Project DOI: 10.21228/M8281N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003220 |
| Study Title | Obesity, sex, and depot drive distinct lipid profiles in murine white adipose tissue |
| Study Summary | To investigate how the lipid composition of the adipose tissue changes with obesity, sex, and depot, we performed comprehensive untargeted lipidomics on the whole adipose tissue of the inguinal and perigonadal adipose depot from lean and obese, male and female mice. The screen allowed us to identify lipid signatures sufficient to differentiate different adipose tissue samples. Such that, increased levels of OxTG and CL, increase in DG and Cer, and increase in HexCer were all able to differentiate adipose tissue based on obesity, sex, or depot, respectively. |
| Institute | University of Utah |
| Last Name | Hilgendorf |
| First Name | Keren |
| Address | EEJMRB 5520 |
| keren.hilgendorf@biochem.utah.edu | |
| Phone | (801) 587-1071 |
| Submit Date | 2023-10-25 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-06-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005280 | AN005281 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6545 QTOF | Agilent 6545 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | pmol/mg tissue | pmol/mg tissue |
MS:
| MS ID: | MS005011 |
| Analysis ID: | AN005280 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | For positive mode, the source gas temperature was set to 225 °C, with a drying gas flow of 11 L/min, nebulizer pressure of 40 psig, sheath gas temp of 350 °C and sheath gas flow of 11 L/min. VCap voltage is set at 3500 V, nozzle voltage 500V, fragmentor at 110 V, skimmer at 85 V and octopole RF peak at 750 V. For negative mode, the source gas temperature was set to 300 °C, with a drying gas flow of 11 L/min, a nebulizer pressure of 30 psig, sheath gas temp of 350 °C and sheath gas flow 11 L/min. VCap voltage was set at 3500 V, nozzle voltage 75 V, fragmentor at 175 V, skimmer at 75 V and octopole RF peak at 750 V. Data processing was performed using Agilent MassHunter (MH) Workstation and software packages MH Qualitiative and MH Quantitative. The pooled QC (n=8) and process blank (n=4) were injected throughout the sample queue to ensure the reliability of acquired lipidomics data. For lipid annotation, accurate mass and MS/MS spectral matching was used with LipidMatch library (Koelmel et al., BMC bioinformatics 18.1 (2017): 1-11.). Results from the positive and negative ionization modes from Lipid Annotator were merged based on the class of lipid identified. Data exported from MH Quantitative was evaluated using Excel where initial lipid targets are parsed based on the following criteria. Only lipids with relative standard deviations (RSD) less than 30% in QC samples are used for data analysis. Additionally, only lipids with background AUC counts in process blanks that are less than 30% of QC are used for data analysis. The parsed excel data tables are normalized based on the ratio to class-specific internal standards prior to statistical analysis. |
| Ion Mode: | POSITIVE |
| MS ID: | MS005012 |
| Analysis ID: | AN005281 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The source gas temperature was set to 225 °C, with a drying gas flow of 11 L/min, nebulizer pressure of 40 psig, sheath gas temp of 350 °C and sheath gas flow of 11 L/min. VCap voltage is set at 3500 V, nozzle voltage 500V, fragmentor at 110 V, skimmer at 85 V and octopole RF peak at 750 V. For negative mode, the source gas temperature was set to 300 °C, with a drying gas flow of 11 L/min, a nebulizer pressure of 30 psig, sheath gas temp of 350 °C and sheath gas flow 11 L/min. VCap voltage was set at 3500 V, nozzle voltage 75 V, fragmentor at 175 V, skimmer at 75 V and octopole RF peak at 750 V. Data processing was performed using Agilent MassHunter (MH) Workstation and software packages MH Qualitiative and MH Quantitative. The pooled QC (n=8) and process blank (n=4) were injected throughout the sample queue to ensure the reliability of acquired lipidomics data. For lipid annotation, accurate mass and MS/MS spectral matching was used with LipidMatch library (Koelmel et al., BMC bioinformatics 18.1 (2017): 1-11.). Results from the positive and negative ionization modes from Lipid Annotator were merged based on the class of lipid identified. Data exported from MH Quantitative was evaluated using Excel where initial lipid targets are parsed based on the following criteria. Only lipids with relative standard deviations (RSD) less than 30% in QC samples are used for data analysis. Additionally, only lipids with background AUC counts in process blanks that are less than 30% of QC are used for data analysis. The parsed excel data tables are normalized based on the ratio to class-specific internal standards prior to statistical analysis. |
| Ion Mode: | NEGATIVE |
