Summary of Study ST003243
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002013. The data can be accessed directly via it's Project DOI: 10.21228/M8DJ7S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003243 |
| Study Title | Lipidomic analysis of serum from WT, liver-specific Gclc KO, liver-specific Nrf2 KO, and liver-specific Gclc-Nrf2 DKO mice. |
| Study Summary | The rate-limiting enzyme in glutathione (GSH) synthesis is Gclc. GSH synthesis is suggested to be implicated in lipogenesis. Loss of GSH synthesis is suggested to increase the activity of NRF2. To examine the impact of GSH and NRF2 on lipid abundance in serum, we performed lipidomic analysis of serum from WT, liver-specific Gclc KO, liver-specific Nrf2 KO, and liver-specific Gclc-Nrf2 DKO mice. |
| Institute | University of Rochester Medical Center |
| Last Name | Harris |
| First Name | Isaac |
| Address | 601 Elmwood Ave, Rochester, New York, 14642-0001, USA |
| isaac_harris@urmc.rochester.edu | |
| Phone | 8572348624 |
| Submit Date | 2024-05-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-06-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005313 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Perkin Elmer Brownlee SPP C18 (75 x 2.1mm, 2.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | Normalized to the median value of total lipid signals |
MS:
| MS ID: | MS005043 |
| Analysis ID: | AN005313 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS2 scan conditions were applied in positive mode, the scan range was from m/z 120–1000, resolution was 120,000 for MS, and 30,000 for DDMS2 (top 10), and the AGC target was 3E6 for full MS and 1E5 for DDMS2, allowing ions to accumulate for up to 200 ms for MS and 50 ms for MS/MS. For MS/MS, the following settings are used: isolation window width 1.2 m/z, stepped NCE at 10, 15, and 25 a.u., minimum AGC 5E2, and dynamic exclusion of previously sampled peaks for 8 s. Quality control (QC) samples were included to check the technical variability and were prepared by mixing an equal volume of lipid extract from each tissue or serum sample. QC samples were included in the analysis sequence every ten samples and monitored for changes in peak area, width, and retention time to determine the performance of the LC-MS/MS analysis. QC samples were subsequently used to align the analytical batches. The lipid peaks were identified, aligned, and exported using MS-DIAL. The data were further normalized to the median value of total lipid signals. Only lipids fully identified by MS2 spectra were included in the analysis. |
| Ion Mode: | POSITIVE |
