Summary of Study ST003257
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002015. The data can be accessed directly via it's Project DOI: 10.21228/M85238 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003257 |
Study Title | Exploration of EMT-dependent changes in the lipidome of KPC cells |
Study Summary | KPC cell lines that are mesenchymal (lines KPC550 and KPC701), mesenchymal/epithelial mixed (lines KPC524 and KPC438), epithelial (lines KPC661 and KPC792) and KPC cell lines with Zeb1 knockout (lines KPCZ519, KPCZ436, KPCZ426) were analyzed for their phospholipid profile by UPLC-MS/MS. Please note that one sample set was measured three times with the same sample-ID, but with different methods (PE, PC, PI), therefore each sub-class has their own raw-data file marked by their corresponding abbreviation (PE, PC, PI; e.g. "200326_Brabletz_EMT_ZEB1_PE_dil_1_10.wiff", "200326_Brabletz_EMT_ZEB1_PC_dil_1_10.wiff" or "200326_Brabletz_EMT_ZEB1_PI_dil_1_10.wiff"). |
Institute | University of Innsbruck |
Department | Michael Popp Institute |
Last Name | Koeberle |
First Name | Andreas |
Address | Mitterweg 24, Innsbruck, Tyrol, 6020, Austria |
Andreas.Koeberle@uibk.ac.at | |
Phone | +43 512 507 57903 |
Submit Date | 2024-05-27 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2024-07-05 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005342 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity H-Class |
Column | Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTRAP |
MS instrument name | ABI Sciex 6500+ |
Ion Mode | NEGATIVE |
Units | relative intensities |
MS:
MS ID: | MS005072 |
Analysis ID: | AN005342 |
Instrument Name: | ABI Sciex 6500+ |
Instrument Type: | QTRAP |
MS Type: | ESI |
MS Comments: | Targeted MRM with pre-optimized settings and subsequent automated integration of selected signals using Analyst 1.6.3 or Analyst 1.7.1 (Sciex). Phospholipids were analyzed in the negative ion mode, and both fatty acid anion fragments were detected by multiple reaction monitoring (MRM). For quantitation, the mean of both transitions was calculated. For the calculation of relative intensities (i.e., the proportion of lipids), all analyzed signals within the subgroup were summarized (= 100%), and the signals of individual lipid species or lipid subfractions are expressed as percentage of this sum. |
Ion Mode: | NEGATIVE |