Summary of Study ST003265
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002027. The data can be accessed directly via it's Project DOI: 10.21228/M8M24P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003265 |
| Study Title | Malic Enzyme 2 maintains metabolic state and anti-tumor immunity of CD8+ T cells |
| Study Summary | For metabolite analysis, naive ME2+/+ and ME2-/- CD8+ T cells were isolated and activated with anti-CD3 (4μg/ml) and anti-CD28 (1μg/ml). Cells were washed twice with ice-cold PBS and metabolites were extracted with ice-cold 80% methanol. The extracts were analyzed by LC-MS/MS. |
| Institute | Peking Union Medical College |
| Last Name | zhang |
| First Name | zhenxi |
| Address | Beijing Dongdan Santiao No.5 |
| 13121716766@163.com | |
| Phone | 69156953 |
| Submit Date | 2024-06-05 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-07-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005349 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Merck SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Focus |
| Ion Mode | UNSPECIFIED |
| Units | Peak area |
MS:
| MS ID: | MS005079 |
| Analysis ID: | AN005349 |
| Instrument Name: | Thermo Q Exactive Focus |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The Q Exactive mass spectrometer was operated in full MS and polarity switching mode (acquisition from m/z 50 to 500) with a scan resolution set at 35,000. Five independent cell cultures were measured for each condition and samples were randomised in order to avoid bias in sample analyses due to machine drift. Q Exactive LC-MS/MS vendor raw data files were converted to the open source format mzML using the program ProteoWizard, and then the XCMS program was used for peak alignment, retention time correction and peak area extraction. Significance was determined using an unpaired Student's t-test. p-value < 0.05 was considered as statistically significant. The acquired spectra were analysed using XCalibur Qual Browser and XCalibur Quan Browser software (Thermo Scientific) by referencing to an internal library of compounds. |
| Ion Mode: | UNSPECIFIED |
