Summary of Study ST003282
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002034. The data can be accessed directly via it's Project DOI: 10.21228/M8PV5M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003282 |
| Study Title | 96-plex metabolomics studies on nutrient-deprived endothelial cells |
| Study Summary | A NeuCode tag which allows for cost efficient incorporation of isotopes is used to enable a 96-plex assay for small molecule metabolomics. A 96-well plate of cell lysates is analyzed simultaneously, providing absolute quantitation of 38 metabolites across 96 samples in a single LC-MS injection. |
| Institute | Saint Louis University |
| Last Name | Armbruster |
| First Name | Michael |
| Address | 4448 Swiss Stone Ln E Apt 3C, Ypsilanti, Michigan, 48197, USA |
| armbrustermr@gmail.com | |
| Phone | 3145501620 |
| Submit Date | 2024-02-15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-07-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005377 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Orbitrap ID-X tribrid |
| Ion Mode | POSITIVE |
| Units | nM |
MS:
| MS ID: | MS005106 |
| Analysis ID: | AN005377 |
| Instrument Name: | Thermo Orbitrap ID-X tribrid |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The IDX was operated with an isolation width of 12 m/z, maximum injection time of 502 ms, AGC of 1e6, positive spray voltage of 3.5 kV, sheath gas of 50 arbitrary units (Arb), aux gas of 10 Arb, sweep gas of 1 Arb, a vaporizer temperature of 350ºC, and a capillary temperature of 325ºC. |
| Ion Mode: | POSITIVE |
