Summary of Study ST001170
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000782. The data can be accessed directly via it's Project DOI: 10.21228/M8M10T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001170 |
Study Title | Timecourse on MCF-7 cells treated with different concentration of doxorubicin |
Study Summary | MCF-7 cells treated with 10μM doxorubicin for 4h followed by subsequent withdrawl of the drug and cultured up to 48h.Doxurubicin-treated cells and control cells were collected at 0,12,24,36,and 48h. Meanwhile,MCF-7 cells continuously exposed to a low dosage of doxorubicin at 0.1μM for 96h. Doxorubicin-treated cells and control cells were collected at 0,24,48,72 and 96h. |
Institute | China Pharmaceutical University |
Last Name | Shao |
First Name | Chang |
Address | Tongjiaxiang #24, Nanjing, Jiangsu, 210000, China |
cici_shao@126.com | |
Phone | 13951628679 |
Submit Date | 2019-04-11 |
Raw Data Available | Yes |
Raw Data File Type(s) | qgd |
Analysis Type Detail | LC-MS |
Release Date | 2020-12-01 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN001935 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Shimadzu LC-30A |
Column | Waters XBridge Amide (100 x 4.6mm,3.5um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | ABI Sciex 5600 TripleTOF |
Ion Mode | NEGATIVE |
Units | normalized peak area |
MS:
MS ID: | MS001791 |
Analysis ID: | AN001935 |
Instrument Name: | ABI Sciex 5600 TripleTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | The MS data was acquired in the negative ion mode using a data-dependent acquisition approach. The electrospray ionization (ESI) source conditions were used as follows: TOF MS scan, m/z 50 – 1000 Da; Product ion scan, m/z 50 – 900 Da; Ion Source Gas 1 (Gas 1), 50 psi; Ion Source Gas 2 (Gas 2), 30 psi; Curtain gas, 30 psi; Source temperature, 500 ℃; Ion Spray Voltage Floating, -4500 V; Declustering potential (DP), -100V; Collision energy (CE), -35 V; CE spread, 10 V. MS data acquisition was controlled by Analyst TF 1.6.1 (AB SCIEX, Framingham, MA, USA). The accurate mass was calibrated by Calibration Delivery System. |
Ion Mode: | NEGATIVE |