Summary of Study ST002165

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001378. The data can be accessed directly via it's Project DOI: 10.21228/M8M12K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002165
Study TitleUntargeted metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours - LCMS Study.
Study TypeUntargeted LCMS
Study SummaryUntargeted metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours. Metabolic profiling of urine samples were performed by TripleTOF 5600+ (AB Sciex) in both ionization mode
Institute
Institute of Nuclear Medicine and Allied Sciences
DepartmentInstitute of Nuclear Medicine and Allied Sciences
LaboratoryMetabolomics facility
Last NameRana
First NamePoonam
AddressDRDO, Lucknow Road, Timarpur
Emailpoonamrana27@gmail.com
Phone+911123905319
Submit Date2021-11-24
Num Groups3
Total Subjects21
Num Males21
Analysis Type DetailLC-MS
Release Date2022-05-31
Release Version1
Poonam Rana Poonam Rana
https://dx.doi.org/10.21228/M8M12K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003547 AN003548
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system ExionLC ExionLC
Column Kinetex EVO C18 (100 x 2.1 mm,1.7um) Kinetex EVO C18 (100 x 2.1 mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name ABI Sciex 5600 TripleTOF ABI Sciex 5600 TripleTOF
Ion Mode POSITIVE NEGATIVE
Units Intensity Intensity

MS:

MS ID:MS003305
Analysis ID:AN003547
Instrument Name:ABI Sciex 5600 TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Samples were ionized using Electron Spray Ionization(ESI) in both the modes. The various MS parameters used for the present study are as discussed. The gas flow, GS1 and GS2 were kept at 50 psi, curtain gas flow (CUR) at 30 psi and capillary voltage(ISVF) at 5500 V for positive and - 4500 V for negative ionization mode with source temperature 400 0C for both ionization modes. The MS calibration was performed through CDS (calibrant delivery system). For MS/MS fragmentation of metabolites, collision energy was set at 35 eV with a collision energy spread of ± 15 eV and declustering potential of 60 eV. Mass range was set at 100- 1200m/z. Metabolites were identified by matching with, company provided library and Metlin public database (https://metlin.scripps.edu).
Ion Mode:POSITIVE
Acquisition Parameters File:MS_Parameter.pdf
  
MS ID:MS003306
Analysis ID:AN003548
Instrument Name:ABI Sciex 5600 TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Samples were ionized using Electron Spray Ionization(ESI) in both the modes. The various MS parameters used for the present study are as discussed. The gas flow, GS1 and GS2 were kept at 50 psi, curtain gas flow (CUR) at 30 psi and capillary voltage(ISVF) at 5500 V for positive and - 4500 V for negative ionization mode with source temperature 400 0C for both ionization modes. The MS calibration was performed through CDS (calibrant delivery system). For MS/MS fragmentation of metabolites, collision energy was set at 35 eV with a collision energy spread of ± 15 eV and declustering potential of 60 eV. Mass range was set at 100- 1200m/z. Metabolites were identified by matching with, company provided library and Metlin public database (https://metlin.scripps.edu).
Ion Mode:NEGATIVE
Acquisition Parameters File:MS_Parameter.pdf
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