Summary of Study ST003006

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001873. The data can be accessed directly via it's Project DOI: 10.21228/M8MF0Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003006
Study TitleBAT metabolomics from cold and TN mouse models
Study SummaryBrown adipose tissue (BAT) metabolites in mice acclimated to cold (6˚C) or thermoneutrality (30˚C) for two weeks. N = 10 for mice housed at 6 ˚C and 9 for mice housed at 30 ˚C.
Institute
Harvard Medical School
Last NameWang
First NameDandan
Address3 Blackfan Circle
Emaildandanwang2022@gmail.com
Phone5083733714
Submit Date2023-12-13
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-02-19
Release Version1
Dandan Wang Dandan Wang
https://dx.doi.org/10.21228/M8MF0Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004937
Analysis type MS
Chromatography type HILIC
Chromatography system Vanquish Horizon
Column Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo orbitrap exploris 240
Ion Mode NEGATIVE
Units Peak area

MS:

MS ID:MS004680
Analysis ID:AN004937
Instrument Name:Thermo orbitrap exploris 240
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:ESI source parameters were set as follows: spray voltage, 3500 V or −2800 V, in positive or negative modes, respectively; vaporizer temperature, 350 °C; sheath gas, 50 arb; aux gas, 10 arb; ion transfer tube temperature, 325 °C. The full scan was set as: orbitrap resolution, 60,000; maximum injection time, 100 ms; scan range, 70–1050 Da. The ddMS2 scan was set as: orbitrap resolution, 30,000; maximum injection time, 60 ms; top N setting, 6; isolation width, 1.0 m/z; HCD collision energy (%), 30; Dynamic exclusion mode was set as auto. The metabolites was quantified by Compound Discoverer 3.3.
Ion Mode:NEGATIVE
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