Summary of Study ST003017
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001878. The data can be accessed directly via it's Project DOI: 10.21228/M8ZQ63 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003017 |
| Study Title | Lipidomics and plasma hormone reveal indicators of reproductive status in Florida manatees (Trichechus manatus latirostris) |
| Study Summary | Florida manatees (Trichechus manatus latirostris) are protected as a threatened species, and data are lacking regarding their reproductive physiology. This study aimed to (1) quantify plasma steroid hormones in Florida manatees from two field sites, Crystal River and Indian River Lagoon, at different gestational stages and to (2) determine the relationship between plasma progesterone concentrations and lipid biochemistry in relation to pregnancy status. Ultra-high performance liquid chromatography-tandem mass spectrometric analysis was used to measure plasma steroid hormones and lipids. Pregnant female manatees were morphometrically distinct from male and non-pregnant female manatees, characterized by larger body weight and maximal girth. Progesterone concentrations in manatees were also elevated during early gestation versus late gestation. Cholesterol, an important metabolic lipid and precursor for reproductive steroids, was not different between groups. Lipidomics quantified 949 lipids and plasma concentrations of a sphingolipid, ceramide non-hydroxy fatty acid-sphingosine and several glycerophospholipids, including lysophosphatidylcholine, phosphatidylethanolamines, plasmenyl-phosphatidylserines and monomethyl phosphatidylethanolamines, were associated with pregnancy status in the Florida manatee. This research contributes to improving knowledge of manatee reproductive physiology by providing data on plasma steroid hormones relative to reproductive status and by assessing how plasma lipids in healthy Florida manatees correspond to progesterone levels. This lipid panel has potential as a diagnostic approach to identify pregnant individuals in fresh and archived samples. These biochemical and morphometric indicators of reproductive status advance the understanding of manatee physiology. |
| Institute | University of Florida |
| Last Name | Brammer-Robbins |
| First Name | Elizabeth |
| Address | 2187 Mowry Rd., Bldg 471 |
| e.brammerrobbins@ufl.edu | |
| Phone | 9104652899 |
| Submit Date | 2023-12-10 |
| Total Subjects | 57 |
| Num Males | 31 |
| Num Females | 26 |
| Publications | https://doi.org/10.1016/j.ygcen.2023.114250 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-06-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004950 | AN004951 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC BEH C18(100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18(100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
MS:
| MS ID: | MS004690 |
| Analysis ID: | AN004950 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All samples were analyzed in full scan positive and negative ion modes with spray voltages +3.0kV and -3.0kV, respectively, at 70,000 resolution at m/z 200, the scan range was m/z 100-1500 and source temperature at 300 °C. Tandem mass spectra were collected via criterion based on data-dependent acquisition for the top-10 ions with stepped normalized collision energy 20, 25, and 30, an isolation window of 1Da, dynamic exclusion of 6 s, and using IE-Omics for the generation of exclusion lists in iterative-exclusion experiments (Koelmel, Kroeger, Gill, et al., 2017). Extracted Lipid Data Analysis LipidMatch software was implemented to identify and integrate the detected lipids (Koelmel, Kroeger, Ulmer, et al., 2017). The peak areas were normalized relative to the internal standards of the same subclass as the lipid of interest. For lipids that did not have an internal standard of the same subclass, the internal standard with the closest chemical structure was used (i.e., internal standard with the same head group). Otherwise, the internal standard with the closest chromatographic retention time (same ionization polarity) was used for analytes without a corresponding internal standard of the same lipid subclass. The plasma lipid concentrations were reported as µg/mL. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | MS Metadata.txt |
| MS ID: | MS004691 |
| Analysis ID: | AN004951 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | All samples were analyzed in full scan positive and negative ion modes with spray voltages +3.0kV and -3.0kV, respectively, at 70,000 resolution at m/z 200, the scan range was m/z 100-1500 and source temperature at 300 °C. Tandem mass spectra were collected via criterion based on data-dependent acquisition for the top-10 ions with stepped normalized collision energy 20, 25, and 30, an isolation window of 1Da, dynamic exclusion of 6 s, and using IE-Omics for the generation of exclusion lists in iterative-exclusion experiments (Koelmel, Kroeger, Gill, et al., 2017). Extracted Lipid Data Analysis LipidMatch software was implemented to identify and integrate the detected lipids (Koelmel, Kroeger, Ulmer, et al., 2017). The peak areas were normalized relative to the internal standards of the same subclass as the lipid of interest. For lipids that did not have an internal standard of the same subclass, the internal standard with the closest chemical structure was used (i.e., internal standard with the same head group). Otherwise, the internal standard with the closest chromatographic retention time (same ionization polarity) was used for analytes without a corresponding internal standard of the same lipid subclass. The plasma lipid concentrations were reported as µg/mL. |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | MS Metadata.txt |
