Summary of Study ST003068
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001913. The data can be accessed directly via it's Project DOI: 10.21228/M8FH9H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003068 |
| Study Title | Attenuation of Helicobacter pylori VacA toxin-induced cell death by modulation of intracellular taurine metabolism _ Study #2 |
| Study Type | untargeted metabolomics analysis |
| Study Summary | AGS cells treated with wild-type VacA have metabolic profiles different from those of AGS cells treated with mutant forms of VacA. Most VacA-induced cellular alterations are attributed to VacA’s ability to form channels within host cell membranes. To determine if the observed VacA-induced metabolic alterations were dependent on VacA’s ability to form membrane channels, we treated AGS cells with the wild-type s1m1 wild-type (WT) toxin (which has robust channel-forming activity), as well as two mutant forms of the toxin, Delta_6-27 or s2m1. VacA Delta_6-27 has a deletion in the VacA amino-terminal hydrophobic region and is defective in channel formation. In the s2m1 mutant toxin, the active s1 isotype sequence of the WT toxin is replaced with the less active s2 sequence, resulting in markedly diminished channel-forming activity. AGS cells treated with the Delta_6-27 or s2m1 mutant VacA did not undergo vacuolation, while AGS cells treated with WT VacA experienced robust vacuolation, consistent with previously published results. |
| Institute | Vanderbilt University |
| Department | Chemistry |
| Laboratory | Center for Innovative Technology |
| Last Name | CODREANU |
| First Name | SIMONA |
| Address | 1234 STEVENSON CENTER LANE |
| SIMONA.CODREANU@VANDERBILT.EDU | |
| Phone | 6158758422 |
| Submit Date | 2024-02-05 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-06-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005025 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Thermo Hypersil GOLD aQ (100 x 2.1mm,1.9um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | time_m/z |
MS:
| MS ID: | MS004764 |
| Analysis ID: | AN005025 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Mass spectrometry raw data was imported, processed, normalized, and reviewed using Progenesis QI v.3.0 (Non-linear Dynamics, Newcastle, UK). |
| Ion Mode: | POSITIVE |
