Summary of Study ST003210

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002001. The data can be accessed directly via it's Project DOI: 10.21228/M8ZJ84 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples  |  Perform analysis on untargeted data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST003210
Study TitleUntargeted metabolomics analysis on single cells and cell clusters of TNBC cell lines using mass spectrometry
Study SummaryTriple-negative breast cancer (TNBC) is more aggressive than other subtypes of breast cancer， and 40% of TNBC patients undergo recurrence and metastasis after treatments. In the metastasis process, clustered cancer cells (cell clusters) exhibited a higher ability of invasion and metastasis. On the other hand, metabolic reprogramming is closely related to the process of cancer metastasis. To explore the metabolic heterogeneity between single cells and cell clusters, mass spectrometry-based untargeted metabolomic analysis was performed on single cells and cell clusters constructed from two TNBC cell lines (MDA-MB-231 and MDA-MB-453). As a result, metabolic profiling between single cells and cell clusters were explored and metabolites contributing to cell clustering were identified.
Institute
Nanjing Medical University
Last NameWang
First NameZhongcheng
Address101Longmian Avenue, Jiangning District, Nanjing 211166, P.R. China
Email1845775254@qq.com
Phone025-86868326
Submit Date2024-04-28
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-05-23
Release Version1
Zhongcheng Wang Zhongcheng Wang
https://dx.doi.org/10.21228/M8ZJ84
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN005262 AN005263 AN005264 AN005265
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Agilent InfinityLab Poroshell 120 HILIC-Z (100 x 2.1mm, 2.7um) Agilent InfinityLab Poroshell 120 HILIC-Z (100 x 2.1mm, 2.7um) Phenomenex Kinetex C18 (100 x 2.1mm,2.6um) Phenomenex Kinetex C18 (100 x 2.1mm,2.6um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Peak area Peak area Peak area Peak area

MS:

MS ID:MS004993
Analysis ID:AN005262
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:All operations and acquisitions were controlled by Xcalibur 2.0.7 (Thermo Fisher Scientific, Bremen, Germany).UPLC-HRMS data were analyzed using MS-DIAL software after conversion to mzML file format using MSConvert (Version 3.0, ProteiWizard).
Ion Mode:POSITIVE
Analysis Protocol File:MS_analysis_protocol.pdf
  
MS ID:MS004994
Analysis ID:AN005263
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:All operations and acquisitions were controlled by Xcalibur 2.0.7 (Thermo Fisher Scientific, Bremen, Germany).UPLC-HRMS data were analyzed using MS-DIAL software after conversion to mzML file format using MSConvert (Version 3.0, ProteiWizard).
Ion Mode:NEGATIVE
Analysis Protocol File:MS_analysis_protocol.pdf
  
MS ID:MS004995
Analysis ID:AN005264
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:All operations and acquisitions were controlled by Xcalibur 2.0.7 (Thermo Fisher Scientific, Bremen, Germany).UPLC-HRMS data were analyzed using MS-DIAL software after conversion to mzML file format using MSConvert (Version 3.0, ProteiWizard).
Ion Mode:POSITIVE
Analysis Protocol File:MS_analysis_protocol.pdf
  
MS ID:MS004996
Analysis ID:AN005265
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:All operations and acquisitions were controlled by Xcalibur 2.0.7 (Thermo Fisher Scientific, Bremen, Germany).UPLC-HRMS data were analyzed using MS-DIAL software after conversion to mzML file format using MSConvert (Version 3.0, ProteiWizard).
Ion Mode:NEGATIVE
Analysis Protocol File:MS_analysis_protocol.pdf
  logo