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MB Sample ID: SA242762
Local Sample ID: | Pyruvate_Soleus_13CNMR-3 |
Subject ID: | SU002515 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 16±3 weeks |
Gender: | Not applicable |
Animal Animal Supplier: | Jackson Labs (Stock # 000664) |
Animal Housing: | Housed in a temperature of 22 oC |
Animal Light Cycle: | 12-hour light/12-hour dark |
Animal Feed: | Ad libitum chow diet food |
Animal Water: | free access to food and water (3-5 animals per cage). |
Select appropriate tab below to view additional metadata details:
NMR:
NMR ID: | NM000261 |
Analysis ID: | AN003949 |
Instrument Name: | Agilent 600 MHz |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-13C |
Field Frequency Lock: | 13C |
Standard Concentration: | 0.5mM DSS |
Spectrometer Frequency: | 600 MHz |
NMR Probe: | HTS-1A |
NMR Solvent: | Phosphate buffer (pH 7.2) + 2 mM EDTA + 0.5 mM DSS + 0.2% of sodium azide in deuterated environment |
NMR Tube Size: | 1.5 mm NMR tube |
Shimming Method: | Varian |
Pulse Sequence: | s2pul |
Water Suppression: | WALTZ-16 |
Pulse Width: | 45 degree |
Receiver Gain: | 60 |
Chemical Shift Ref Cpd: | DSS |
Temperature: | 30oC |
Number Of Scans: | 6000 to 30000 scans |
Dummy Scans: | 8 |
Acquisition Time: | 1.5 s |
Relaxation Delay: | 1.5 s |
Spectral Width: | 36764.7 Hz |
Num Data Points Acquired: | 55147 |
Real Data Points: | 65536 |
Line Broadening: | 0.5 Hz |
Zero Filling: | 65,536 points |
Apodization: | Exponential |
Baseline Correction Method: | Whittaker Smoother |
Chemical Shift Ref Std: | 0 ppm for DSS |