Summary of Study ST000051
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000049. The data can be accessed directly via it's Project DOI: 10.21228/M8W88R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000051 |
Study Title | Fetal metabolomic signature of exposure to iAs during pregnancy |
Study Type | Metabolomics |
Study Summary | This metabolomics pilot study was aimed to identify a fetal metabolomic signature of exposure to iAs during pregnancy. Fetal cord blood serum was collected immediately after delivery from a saline cleaned umbilical cord using an anticoagulant-free vacutainer tube, after clot formation the tube was centrifuged at 1200 rpm and the serum was collected and stored at -70°C. A subset of 50 cord serum samples were selected from the larger BEAR cohort to represent a wide range of iAs exposure as determined by iAs in drinking water (DW-iAs). The exposure during pregnancy was confirmed using U-tAs. A total of 50 cord blood serum samples were used in the metabolomics analysis The samples included 25 newborns with lower maternal iAs exposure levels (DW < 25?g As/L, mean U-tAs=16 ?g/L) and 25 newborns with higher maternal iAs exposure levels (DW> 25?g As/L, mean U-tAs =107 ?g/L). |
Institute | University of North Carolina |
Department | Systems and Translational Sciences |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2014-04-30 |
Num Groups | 2 |
Total Subjects | 50 |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Uploaded File Size | 31 M |
Analysis Type Detail | NMR |
Release Date | 2018-08-27 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
NMR ID: | NM000024 |
Analysis ID: | AN000087 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Field Frequency Lock: | Deuterium |
Standard Concentration: | 1 mM |
Spectrometer Frequency: | 950 MHz |
NMR Probe: | 5 mm Inverse ATMA |
NMR Solvent: | D2O |
NMR Tube Size: | 3 mm, 4 inches |
Shimming Method: | Topshim |
Pulse Sequence: | cpmgpr1d |
Water Suppression: | presat |
Pulse Width: | 8.06 s |
Power Level: | 12.589W |
Receiver Gain: | 4 |
Offset Frequency: | 4469.49 Hz |
Chemical Shift Ref Cpd: | Formate |
Temperature: | 298 K |
Number Of Scans: | 256 |
Dummy Scans: | 16 |
Relaxation Delay: | 2 s |
Spectral Width: | 19531.25 Hz |
Num Data Points Acquired: | 65523 |
Real Data Points: | 65536 |
Line Broadening: | 0.5 |
Zero Filling: | Yes |
Apodization: | Lorentzian |
Baseline Correction Method: | Polynomial |
Chemical Shift Ref Std: | Formate |