Summary of Study ST001616
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001039. The data can be accessed directly via it's Project DOI: 10.21228/M8D400 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001616 |
Study Title | Unique metabolomic profile of skeletal muscle in chronic limb threatening ischemia (aqueous phase samples ) |
Study Type | NMR |
Study Summary | This project is focused on a cross-sectional analysis of non-PAD controls and CLTI patients undergoing either a vascular intervention or undergoing limb amputation was performed and involved a detailed assessment of the limb muscle metabolome using HR-MAS and solution state NMR spectroscopy. It was hypothesized that patients undergoing limb amputation would present with altered muscle metaolite features compared with non-PAD controls. |
Institute | University of Florida |
Department | Applied Physiology and Kinesiology |
Laboratory | Rm 42 and Rm 43 |
Last Name | Ryan |
First Name | Terence |
Address | University of Florida, Applied Physiology and Kinesiology, 1864 stadium RD, Gainesville, FL 32611 |
ryant@ufl.edu | |
Phone | 352-294-1700 |
Submit Date | 2020-11-30 |
Num Groups | 3 |
Total Subjects | 30 |
Num Males | 25 |
Num Females | 5 |
Study Comments | PAD metabolomic study via NMR. A detailed assessment of the limb muscle metabolome using semi-solid and solution state NMR spectroscopy |
Publications | Journal of Clinical Medicine |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2021-05-30 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
NMR ID: | NM000188 |
Analysis ID: | AN002651 |
Instrument Name: | Bruker Avance Neo 600 MHz/54mm console |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Field Frequency Lock: | Deuterium |
Standard Concentration: | 0.5 mM DSS |
Spectrometer Frequency: | 600.2328273 MHz |
NMR Probe: | 1.7 mm TXI CryoProbe |
NMR Solvent: | Phosphate buffer (pH 7.2) + 2 mM EDTA + 0.5 mM DSS + 0.2% of sodium azide in deuterated environment |
NMR Tube Size: | 1.7 mm O.D. |
Shimming Method: | Topshim |
Pulse Sequence: | noesypr1d |
Water Suppression: | presat |
Pulse Width: | 90-degree |
Receiver Gain: | 101 |
Offset Frequency: | 2827.31 Hz |
Chemical Shift Ref Cpd: | DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid) |
Temperature: | 25 o C |
Number Of Scans: | 128 scans |
Dummy Scans: | 8 |
Acquisition Time: | 4 s |
Relaxation Delay: | 1 s |
Spectral Width: | 7142.9 Hz |
Num Data Points Acquired: | 28571 |
Real Data Points: | 65536 |
Line Broadening: | 0.22 Hz |
Zero Filling: | 65,536 points |
Apodization: | Exponential |
Baseline Correction Method: | Spline |
Chemical Shift Ref Std: | 0 ppm for DSS |