Summary of Study ST003286

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002038. The data can be accessed directly via it's Project DOI: 10.21228/M85V5Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST003286
Study TitleInteraction between NSCLC cells, CD8+ T cells and immune checkpoint inhibitors potentiates coagulation and promotes metabolic remodeling- new cues on CAT-VTE
Study SummaryCancer-related thrombosis (CAT) and venous thromboembolism (VTE) are common cancer-related sequelae linked with high mortality, therefore finding predictive indicators is critical. Immune checkpoint inhibitors (ICI) are utilized in cancer immunotherapy to activate T lymphocytes against cancer cells. Recent retrospective investigations found that some individuals experienced increased VTE after receiving ICI. We postulated that ICI (anti-CTLA4 and anti-PD1) effect on immunological and cancer cells, as well as their interaction, contributes for an elevated risk of CAT-VTE. Our objective is to investigate this molecular interaction in order to uncover potential prothrombotic indicators. We pharmacologically modulated non-small cell lung cancer (NSCLC) cell lines in co-culture with CD8+ T lymphocytes (T CD8+) obtained from healthy blood donors. Nuclear Magnetic Resonance (NMR) metabolic remodeling analysis revealed differences in extracellular metabolite concentrations after T CD8+ and ICI treatment.
Institute
ITQB NOVA
Last NameGonçalves
First NameLuís
AddressAvenida Republica
Emaillgafeira@itqb.unl.pt
Phone214469464
Submit Date2023-11-20
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2024-07-03
Release Version1
Luís Gonçalves Luís Gonçalves
https://dx.doi.org/10.21228/M85V5Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

NMR:

NMR ID:NM000285
Analysis ID:AN005383
Instrument Name:Bruker Ultrashield 500 Plus
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
NMR Comments:Spectra were acquired in a magnetic field of 500 MHz in an Ultrashied 500 Plus spectrometer (Bruker) with a TCI-Z probe at Instituto de Tecnologia Química e Biológica da Universidade NOVA de Lisboa (ITQB-UNL), Oeiras. The spectra analysis was made in TopSpin 4.1 software, according to Chenomx NMR suite 8.11 and Human Metabolome Database (HMDB).
Spectrometer Frequency:500 MHz
NMR Probe:5 mm prodigy TCI-z
NMR Solvent:10% D2O/90% H20
NMR Tube Size:5 mm
  logo