Summary of Study ST000591
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000432. The data can be accessed directly via it's Project DOI: 10.21228/M86W3V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000591 |
Study Title | Metablomic profiling in acc1-5 mutant and wild type arabidiopsis |
Study Summary | This experiment tests the metabolic consequence of a mutation at the ACC1 gene (At1g36160). The allele of acc1-5 bearing an EMS mutation, which cause a single amino acid substitution from aspartic acid to asparagine. Seedlings from both the acc1-5 mutant and the wild type were harvested and analyzed via HILIC LC-MS. Of particular interest are metabolites which would be affected by depletion of malonyl-CoA pools (flavenoids) and primary metabolites. |
Institute | Agriculture and Agri-Food Canada |
Department | London Research and Development Centre |
Laboratory | Renaud |
Last Name | Renaud |
First Name | Justin |
Address | 1391 Sandford street, London, Ontario, Canada |
justin.renaud@agr.gc.ca | |
Phone | 519-953-6698 |
Submit Date | 2017-03-12 |
Publications | Chen, Chen, et al. "Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Arabidopsis." Nature Plants (2017): 1. |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-25 |
Release Version | 1 |
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Project:
Project ID: | PR000432 |
Project DOI: | doi: 10.21228/M86W3V |
Project Title: | Understanding the effects of acetyl-CoA carboxylase (ACC1) upon the metabolite profiles of Arabidopsis |
Project Type: | Single gene knockout impact on Arabidopsis metabolites |
Project Summary: | The arabidopsis gene, acetyl-CoA carboxylase1 (ACC1) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA. When this gene malfunctions, there are elevated levels of acetyl-CoA which have been shown to increase levels of histone acetylation. This project aims to understand how a malfunctioning ACC1 effects the levels of primary metabolites by comparing to metabolite profiles of wild type Arabidopsis grown under identical conditions. |
Institute: | Agriculture and Agri-Food Canada |
Department: | Chemistry |
Laboratory: | Renaud |
Last Name: | Renaud |
First Name: | Justin |
Address: | 1391 Sandford Street |
Email: | justin.renaud@agr.gc.ca |
Phone: | 519-953-6698 |
Funding Source: | Natural Science and Engineering Research Council of Canada (R4019A01) and Agriculture and Agri-Food Canada A-base |