Summary of Study ST001393
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000956. The data can be accessed directly via it's Project DOI: 10.21228/M84386 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001393 |
Study Title | Sea-ice diatom compatible solute shifts |
Study Type | Compatible solutes were quantified in sea-ice diatoms |
Study Summary | Sea-ice algae provide an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures of the Antarctic sea-ice diatom, Nitzschia lecointei, displayed large differences in their metabolomes when grown in a matrix of conditions that included temperatures of –1 and 4°C, and salinities of 32 and 41, despite relatively small changes in growth rate. Temperature exerted a greater effect than salinity on cellular metabolite pool sizes, though the N- or S-containing compatible solutes, 2,3-dihydroxypropane-1-sulfonate (DHPS), glycine betaine (GBT), dimethylsulfoniopropionate (DMSP), and proline responded strongly to both temperature and salinity, suggesting complexity in their control. We saw the largest (> 4 fold) response to salinity for proline. DHPS, a rarely studied but potential compatible solute, reached the highest intracellular compatible solute concentrations of ~ 85 mM. When comparing the culture findings to natural Arctic sea-ice diatom communities, we found extensive overlap in metabolite profiles, highlighting the relevance of culture-based studies to probe environmental questions. Large changes in sea-ice diatom metabolomes and compatible solutes over a seasonal cycle could be significant components of biogeochemical cycling within sea ice. |
Institute | University of Washington |
Department | School of Oceanography |
Laboratory | Ingalls Lab |
Last Name | Dawson |
First Name | Hannah |
Address | 1501 NE Boat Street, Marine Science Building, Room G, Seattle, WA 98195 |
hmdawson@uw.edu | |
Phone | 2062216750 |
Submit Date | 2020-03-24 |
Publications | Dawson et al., Elementa |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2020-09-29 |
Release Version | 1 |
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Project:
Project ID: | PR000956 |
Project DOI: | doi: 10.21228/M84386 |
Project Title: | Sea-ice diatom compatible solute shifts |
Project Type: | Marine Metabolomics |
Project Summary: | Sea-ice algae provide an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures of the Antarctic sea-ice diatom, Nitzschia lecointei, displayed large differences in their metabolomes when grown in a matrix of conditions that included temperatures of –1 and 4°C, and salinities of 32 and 41, despite relatively small changes in growth rate. Temperature exerted a greater effect than salinity on cellular metabolite pool sizes, though the N- or S-containing compatible solutes, 2,3-dihydroxypropane-1-sulfonate (DHPS), glycine betaine (GBT), dimethylsulfoniopropionate (DMSP), and proline responded strongly to both temperature and salinity, suggesting complexity in their control. We saw the largest (> 4 fold) response to salinity for proline. DHPS, a rarely studied but potential compatible solute, reached the highest intracellular compatible solute concentrations of ~ 85 mM. When comparing the culture findings to natural Arctic sea-ice diatom communities, we found extensive overlap in metabolite profiles, highlighting the relevance of culture-based studies to probe environmental questions. Large changes in sea-ice diatom metabolomes and compatible solutes over a seasonal cycle could be significant components of biogeochemical cycling within sea ice. |
Institute: | University of Washington |
Department: | School of Oceanography |
Laboratory: | Ingalls Lab |
Last Name: | Dawson |
First Name: | Hannah |
Address: | 1501 NE Boat Street, Marine Science Building, Room G, Seattle, WA 98195 |
Email: | hmdawson@uw.edu |
Phone: | 2062216750 |
Funding Source: | Booth Foundation, NSF, UW Graduate Top Scholar Award, Gordon and Betty Moore Foundation |
Publications: | Dawson et al., Elementa |