Summary of Study ST003282
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002034. The data can be accessed directly via it's Project DOI: 10.21228/M8PV5M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003282 |
Study Title | 96-plex metabolomics studies on nutrient-deprived endothelial cells |
Study Summary | A NeuCode tag which allows for cost efficient incorporation of isotopes is used to enable a 96-plex assay for small molecule metabolomics. A 96-well plate of cell lysates is analyzed simultaneously, providing absolute quantitation of 38 metabolites across 96 samples in a single LC-MS injection. |
Institute | Saint Louis University |
Last Name | Armbruster |
First Name | Michael |
Address | 4448 Swiss Stone Ln E Apt 3C, Ypsilanti, Michigan, 48197, USA |
armbrustermr@gmail.com | |
Phone | 3145501620 |
Submit Date | 2024-02-15 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-07-15 |
Release Version | 1 |
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Project:
Project ID: | PR002034 |
Project DOI: | doi: 10.21228/M8PV5M |
Project Title: | High Throughput Metabolomics using 96-plex Isotope Tagging |
Project Summary: | Chemical tags with 96 unique masses are reported which alleviate the metabolomic workflow bottleneck and allow for absolute quantitation. A metabolic screen for carboxylic acids was performed on mammalian cells deprived of various nutrients and showed 24% RSD and analysis of 288 samples in 2 hours. |
Institute: | Saint Louis University |
Department: | Chemistry |
Last Name: | Armbruster |
First Name: | Michael |
Address: | 3501 Laclede Ave, St Louis, MO 63103 |
Email: | armbrustermr@gmail.com |
Phone: | 3145501620 |
Funding Source: | 5R01GM134081 |