Summary of Study ST003282

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002034. The data can be accessed directly via it's Project DOI: 10.21228/M8PV5M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003282
Study Title96-plex metabolomics studies on nutrient-deprived endothelial cells
Study SummaryA NeuCode tag which allows for cost efficient incorporation of isotopes is used to enable a 96-plex assay for small molecule metabolomics. A 96-well plate of cell lysates is analyzed simultaneously, providing absolute quantitation of 38 metabolites across 96 samples in a single LC-MS injection.
Institute
Saint Louis University
Last NameArmbruster
First NameMichael
Address4448 Swiss Stone Ln E Apt 3C, Ypsilanti, Michigan, 48197, USA
Emailarmbrustermr@gmail.com
Phone3145501620
Submit Date2024-02-15
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-07-15
Release Version1
Michael Armbruster Michael Armbruster
https://dx.doi.org/10.21228/M8PV5M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR002034
Project DOI:doi: 10.21228/M8PV5M
Project Title:High Throughput Metabolomics using 96-plex Isotope Tagging
Project Summary:Chemical tags with 96 unique masses are reported which alleviate the metabolomic workflow bottleneck and allow for absolute quantitation. A metabolic screen for carboxylic acids was performed on mammalian cells deprived of various nutrients and showed 24% RSD and analysis of 288 samples in 2 hours.
Institute:Saint Louis University
Department:Chemistry
Last Name:Armbruster
First Name:Michael
Address:3501 Laclede Ave, St Louis, MO 63103
Email:armbrustermr@gmail.com
Phone:3145501620
Funding Source:5R01GM134081
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